analytica-acta-2016_posters-accepted-abstracts

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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
J Anal Bioanal Tech 2016, 7:5(Suppl)
DNA binding study of Co (III) complexes of hydroxamic acid and 1, 10-phenanthroline complex
F Khan and Rakesh K Sahu
NIT Raipur, India
Alarge number of transition metal complexes have been used as cleavage agents of DNA and also for novel potential DNA-
targeted antitumor drugs. This is essential for further expected applications in many areas like biological gene regulators
or cancer chemotherapeutic agents. The identification of metal complex–DNA interaction is of fundamental importance
to understanding the molecular basis of therapeutic activity. Metal complexes are well known to accelerate the drug action
and the efficiency of a therapeutic agent can often be enhanced upon coordination with metal ions. The pharmacological
activity has also been found to be highly dependent on the nature of the metal ion and the donor sequence of the ligands,
as different ligands exhibit different biological properties. In the present work a new Co (III) complexes of mixed ligands, 1,
10-phenenthroline (phen) and hydroxamic acid (L1 = AHA (acetohydroxamic acid), and L2 = BHA (benzohydroxamic acid)
were synthesised and characterized by NMR, IR, UV-visible and elemental analysis. In the complexes, [Co(phen)2Ln] (Ln =
L1 , L2), the metal ion is coordinated by six atoms, two oxygen atom from hydroxamic acid and 4N atom from co-ligand 1,
10-phenenthroline to form octahedral Co(III) complexes. The interaction of these complexes with calf thymus DNA has been
investigated by absorption spectroscopy and fluorescence spectroscopy.
The effect of pH on the partitioning of polychlorinated biphenyls (PCBs) between sediment grain
sizes and water
Gbadebo Clement Adeyinka and Brenda Moodley
University of KwaZulu-Natal, South Africa
In natural environmental media organic pollutants (OPs) undergo partitioning with particulate matter (PM) and dissolved
organic carbon (DOC) in water, and with surface sites on the sediment particle material with which the water is in contact.
The sorption of organic pollutants within the environmental media is one of the significant factors affecting (in most cases
controlling) the distribution, transportation and fate of the pollutant as well as the remediation of polluted water within the
aquatic ecosystem. The aim of this research was to investigate the effects of pH on the phase partitioning of organic pollutants
such as PCB congeners between the aqueous phase and various modeled sediment grain sizes. A model sample of sediment
was sieved into five grain sizes, <75 μm, 100 μm, 200 μm, 300 μm and 425 μm, respectively. The surface areas as well as pore
volume distributions of the different sediment grain sizes were determined using BET-adsorption/desorption isotherms. The
surface areas as shown by BET-adsorption isotherms were between 0.688-14.361 m2/g (425 µm-75 µm). The organic carbon
content was determined using the Walkley Black method. The organic carbon content was found to be greater on the smaller
sediment grain size of 75 μm and least in the 425 μm grain size (2.94 to 11.43 %). Laboratory batch experiments were used to
evaluate the adsorption/desorption as well as the adsorption ratio of the soil/sediment (Kd) within the different sediment grain
sizes. The results showed that soil grain size of <75 μm showed the most adsorption of PCB congeners, in the range of 79.90-
89.97 % while a sediment grain size of 300 μm showed lowest adsorption between 32.60-64.14 %. This could be attributed to
the fact that sediment grain size (75 µm,) with high surface area adsorbed more of the analytes compared to other sediment
grain sizes. Also, the higher organic content associated with the 75 μm grain size further allows for more partitioning of the
organic pollutant to the sediment. The effect of pH on the sorption capacity of the sediment grain sizes was also studied. A pH
of 6.5 showed the highest percentage adsorption. This was due to the non-polar PCBs preferring to partition to the sediment
at near neutral pH.