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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
J Anal Bioanal Tech 2016, 7:5(Suppl)
Development, validation and application of RP-HPLC method: Simultaneous determination of
antihistamine and preservatives with Paracetamol in liquid formulations and human serum
Najmul Hasan1, Mathurot Chaiharn2, Zulfiqar Ali Mirani3, Umair Ali Toor1, Nawab Sher4 and Farhan Ahmed Siddiqui 5
1Hallym University, South Korea
2Maejo University, Thailand
3Pakistan Council of Scientific and Industrial Research Laboratories Complex, Pakistan
4University of Karachi, Pakistan
5Federal Urdu University, Pakistan
In this article we described the development and validation of stability indicating, accurate, precise and simple ion-pairing
RP-HPLC method for simultaneous determination of paracetamol and cetirizine HCl along with preservatives i.e.,
propylparaben and methylparaben in pharmaceutical dosage forms of oral solution and in serum. Acetonitrile: buffer: sulfuric
Acid (45:55:0.3 v/v/v) was the mobile phase at flow rate 1.0 mL min-1 using a Hibar® Lichrosorb® C18 column and monitored
at wavelength of 230 nm. The averages of the absolute and relative recoveries were found to be 99.3%, 99.5%, 99.8% and
98.7% with correlation coefficient of 0.9977, 0.9998, 0.9984, and 0.9997 for cetirizine HCl, paracetamol, methylparaben and
propylparaben, respectively. The limit of quantification and limit of detection were in range of 0.3-2.7 ng mL-1 and 0.1-0.8 ng
mL-1, respectively. Under stress conditions of acidic, basic, oxidative, and thermal degradation, maximum degradation was
observed in basic and oxidative stress where a significant impact was observed, while all drugs were found almost stable in the
other conditions. The developed method was validated in accordance to ICH and AOAC guidelines. The proposed method was
successfully applied to quantify the amount of paracetamol, cetirizine HCl and two most common microbial preservatives in
bulk, dosage form and physiological fluid.
Application of linear voltammetry in the determination of vitamin C in peppers
Hugo Romero1, Carlos Garcia1, Kevin Noles 1, Lenys Fernandes2 and Kerly Davila1
1Universidad Tecnica de Machala, Ecuador
2Universidad Simon Bolivar, Venezuela
The main objective of this research was to compare the content of ascorbic acid peppers (Capsicum annuum), in state of
ripening green and red of the same variety. Pepper extract was obtained by crushing the fruit in a porcelain mortar, the
liquid obtained was filtered on filter paper. For determining the content of ascorbic acid is used linear sweep voltammetry
using three electrodes; as working electrode a platinum wire of high purity, glassy carbon as working electrode and reference
electrode Ag/AgCl/KCl. A pattern of ascorbic acid and a solution of HNO3 and 0.1M NaNO3 as supporting electrolyte were
used for the calibration curve. The results indicate that, green pepper has an average concentration of 1680 ppm of ascorbic
acid, and red pepper reached a value of 2283 ppm. These results are superior to those obtained by Chaves et al., in pepper by
HPLC. In this case, values of 1000 ppm to 1500 ppm for green pepper and red pepper were found respectively.