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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
J Chromatogr Sep Tech 2016, 7:5(Suppl)
The possibilities of amino acid ionic liquids as a chiral selectors at separation of enantiomers of amino
acids and β-blockers
E A Kolobova, L A Kartsova, E A Bessonova, E V Alopina and E Safonova
Saint-Petersburg State University, Russia
C hiral separation is an important subject in science as well as in technology. Various chiral selectors, such as cyclodextrins,
antibiotics and crown ethers have been widely used in separation of enantiomers because of their chiral recognition
abilities. However, the application of many current chiral selectors is often limited due to their low solubility, difficult synthesis,
thermal instability as well as high cost. In addition, most of selectors need to be dissolved in other solvents or in a solvent
system as work solution. Therefore, using chiral ionic liquids as chiral selector is promising. Amino acids ionic liquids [C4Mim]
[L-Pro], [C8Mim][L-Pro], [C12Mim][L-Pro] were synthesized and characterized by NMR-spectrums. In the course of the
optimization of chiral separation conditions were varied: the composition background electrolyte, the structure of selectors
and the concentration of ionic liquid in the modified buffer electrolytes. IL [C4Mim][L-Pro] was the most effective ionic liquid
for the chiral amino acids separation under ligand-exchange capillary electrophoresis. The highest enantioselectivity factors
(α=5.2) were achieved for tryptophane when complexing metal was cupper. Synergetic effect was observed at simultaneously
addition 2-OH-proplyl-β-cyclodextrin and chiral IL to the running buffer as a result enantiomers of propranolol and carvedilol
were separated. Analysis of drug formulation “Carvedilol zentiva” and “Anaprilin” was performed.
New pre-concentration techniques in capillary electrophoresis for determination of bioactive
compounds in complex mixtures
L A Kartsova, E A Bessonova, V F Gallyamova, A V Nikolaev and E V Obedkova
Saint-Petersburg State University, Russia
Our report is focused on the development of new analytical approaches for electrophoretic determination of biologically
active substances with traditional CE and developed microfluidic chip-analyzer with different variants of on-line pre-
concentration. The use of hypercrosslinked polystyrene as a sorption material in the preparation of urine and blood serum
for analysis provided the decrease in detection limits for hydrophobic and hydrophilic analytes. Application of water-
soluble oligosaccharide derivatives hyperbranched polyethylenimine (PEI) as a covalent coating of silica fused capillary
and combination of focusing principles of different variants on-line pre-concentration for analysis of proteins resulted in a
1100-fold improvement in sensitivity. The potential of long chain ionic liquids for on-line sample concentration techniques of
ionogenic and neutral analytes in biological objects by different modes of capillary electrophoresis: zone (CZE) and micellar
(MEKC) modes with normal and reversed polarity were investigated. The compounds chosen were biogenic amines and steroid
hormones. Imidazolium-based ionic liquids C12 MImCl, C 16MImCl were used both as modifiers of electrophoretic systems and
as pseudostationary phase. Sweeping with C16MImCl micelles in BGS has provided 83-112-fold sensitivity enhancement
factors for catecholamines. It was found out that using highly conductivity sample matrix in sweeping leads to a significant
increase in efficiency of analytes up to 1∙106 t.p. Chemometric processing of the obtained characteristic profiles of biologically
active analytes of blood serum and urine samples from healthy donors and patients with endocrine diseases proved to be
informative as an additional diagnostic criteria.