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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
J Chromatogr Sep Tech 2016, 7:5(Suppl)
Prediction of fetal lung maturity using L/S ratio analysis with a simplified sample preparation, using
a commercial microtip-column combined with mass spectrometric analysis
Hee-Jung Chung
Dankook University, South Korea
Fetal lung maturity is estimated using the lecithin/sphingomyelin ratio (L/S ratio) in amniotic fluid and it is commonly
measured with thin-layer chromatography (TLC). The TLC method is time consuming and technically difficult; however,
it is widely used because there is no alternative. We evaluated a novel method for measuring the L/S ratio, which involves a
tip-column with a cation-exchange resin and mass spectrometry. Phospholipids in the amniotic fluid were extracted using
methanol and chloroform. Choline-containing phospholipids such as lecithin and sphingomyelin were purified by passing
them through the tip-column. LC-MS/MS and MALDI-TOF were used to directly analyze the purified samples. The L/S ratio
by mass spectrometry was calculated from the sum peak intensity of the six lecithin, and that of sphingomyelin 34:1. In 20
samples, the L/S ratio determined with TLC was significantly correlated with that obtained by LC-MS/MS and MALDI-TOF.
There was a 100% concordance between the L/S ratio by TLC and that by LC-MS/MS (kappa value=1.0). The concordance
between the L/S ratio by TLC and that by MALDI-TOF was also 100% (kappa value=1.0). Our method provides a faster,
simpler, and more reliable assessment of fetal lung maturity. The L/S ratio measured by LC-MS/MS and MALDI-TOF offers a
compelling alternative method to traditional TLC.
Isolation of ulceroprotective cucurbitane type triterpenoids from Cucumis melo seeds
Gurpreet Singh Bal and Naresh Singh Gill
Punjab Technical University, India
Medicinal plants are the richest bio-resources of drugs in traditional medicinal systems, modern medicines, folk
medicines, intermediate and chemicals entitled for synthetic drugs. Plants provide a source of inspiration for novel drug
development as they contain a vast array of substances that treat chronic diseases. Cucumis melo seeds have been traditionally
used for treating various health ailments. The main aim of our current study is to isolate cucurbitane-type triterpenoids from
Cucumis melo seed extract and conduct anti-ulcerogenic activity of the isolated compound. Phytochemical investigations of
methanolic seed extract of Cucumis melo was carried out which showed the presence of various important phytoconstituents.
The main active constituents of Cucumis melo have shown a number of potent pharmacological activities. The isolation of
Cucurbitane-type triterpenoids was carried out by column chromatography using methanolic seed extract of Cucumis melo.
Mobile phase hexane and hexane-ethyl acetate (98:2) was used to run the column. TLC profiling was done simultaneously
in an appropriate solvent system (hexane: ethyl acetate, 97:3). Various fractions were collected. The fractions with similar Rf
value were pooled together. Fractions giving single spot in the TLC were regarded as pure. The isolated compound showed
positive result for Liebermann-Burchard test from which we can conclude that the isolated compound might be triterpenoid.
The structure of the isolated compound was determined by IR, 1HNMR, 13CNMR techniques. The spectral analysis of the
isolated compound showed following results: IR- it showed the peaks at 3383, 2976, 2814, 1721, 1465, 1123 cm-1 indicated the
presence of alcoholic group.