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Journal of Chromatography & Separation Techniques | Volume: 09
8
th
World Congress on Chromatography
September 13-14, 2018 | Prague, Czech Republic
Polymer Science and Technology
4
th
International Conference on
Joint Event on
&
Miroslav Ryska
Quinta-Analytica s.r.o.,
Czech Republic
T
he source of the “Matrix effect” as a consequence of analyte ions suppression
or ions enhancement must be sought in the presence of unknown impurities
from matrix. They are participating in the complex ionization process in parallel
or competing ion-molecular reactions. Not only impurities from extracts but
impurities adsorbed in the ion source and/or in the analytical system may play
an important role. These adsorbed substances cannot be fully removed from the
system by any cleaning procedure.
To fully compensate for the negative impact of the “Matrix effect“, use of
isotopically labeled internal standards (isotope dilution technique) proved to be
the only effective technique. This applies especially to LC/MS/MS determination
of drugs and their metabolites in complex extracts of biological matrices. The
isotope dilution technique is successful regardless of the method of purification,
the ionization technique (APCI or ESI) and the type of the equipment used. The
isotope dilution technique proved to be 100% effective for the compensation of
matrix effect influences in 181 analytical methods developed and validated. The
strict requirements of EMA guidelines to investigate different plasma sources
for the assessment of the matrix effect in the analytical method validation are
discussed.
Miroslav Ryska, J Chromatogr Sep Tech 2018, Volume: 09
DOI: 10.4172/2157-7064-C2-042
The Use of Isotopiocally Labeled Internal Standards in Quantitative LC/MS – TheWay of The Full
Compensation of Negative Impact of “Matrix Effect”
Biography
Miroslav Ryska (1938) holds an undergraduate degree from Charles University, along with an M.S. in Physical Chemistry from Moscow State University and a
PhD. from the Institute of Macromolecular Chemistry of Czechoslovak Academy of Sciences. From 1961 to 1978 he worked at the Institute of Macromolecular
Chemistry of the Czechoslovak Academy of Sciences. In 1978 - 1997 he worked as a researcher in the field of MS and its application in research of metabolism
and pharmacokinetics of drugs at the Research Institute for Pharmacy and Biochemistry in Prague. He has written more than 100 publications mainly on the topic of
mass spectrometry, trace analyses, analyses of drugs, metabolites and quantitative analysis. In the 1990’s Mr. Ryska acted as an Editor of two international journals,
The Journal of Mass Spectrometry and Rapid Communication in Mass Spectrometry. He founded Quinta-Analytica
s.r.o.in1997.
miroslav.ryska@quinta.cz