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conferenceseries
.com
Volume 8, Issue 10 (Suppl)
J Earth Sci Clim Change, an open access
ISSN: 2157-7617
Climate Change 2017
October 19-21, 2017
CLIMATE CHANGE
October 19-21, 2017 | Rome, Italy
4
th
World Conference on
Temperature effects on acetaminophen toxicity using medaka
Chisato Kataoka, Takahiro Sugiyama, Hikaru Kitagawa
and
Shosaku Kashiwada
Toyo University, Japan
A
cetaminophen (n-acetyl-p-aminophenol, AAP) is one of the most widely used over-the-counter drugs for relief of
fever and headaches due to its analgesic and antipyretic properties, and a major ingredient in cold/flu remedies. AAP is
generally considered safe for human; however, effects of AAP on aquatic organisms is unknown. Release waste including AAP
into aquatic environment can pose an ecological risk. Fish is major organisms in aquatic ecosystems and their habitats are
classified by temperature. Their physiological activity should be affected by temperature. Hence, toxic effects of AAP on fish
will be temperature-dependent. We employed medaka (Oryzias latipes) as a model to see temperature effects on APP toxicity
because they have a wide range of temperature-tolerance (4
0
C to 35
0
C). Medaka larvae were exposed to 50 mg/L of AAP under
15
0
C, 25
0
C (optimum temperature) or 30
0
C for 4 days. Another group of medaka larvae was exposed to 0 mg/L of AAP under
same condition as a control. On day 4, full body length of larvae, heart rate (beats/15 s), and relative ATP value were measured.
Full body length of larvae and relative ATP value at 30
0
C were significantly decreased by AAP exposure, and heart rate at 15
0
C
was significantly decreased by AAP exposure compared with each temperature control. Then, another group of medaka was
exposed to an increased concentration of AAP (150 mg/L) under the same condition. On day 4, the exposed medaka was
subjected to hematological analysis and histological analysis. Hematological analysis revealed AAP exposure increased ratio of
a deformed red blood cell with increasing temperature. In addition, red blood cells distributed in gills were notably decreased
by AAP at 30
0
C. Histological analysis of liver showed AAP exposure increased ratio of vacuole with increasing temperature.
These data suggested increased temperature caused enhancement of AAP toxicities in medaka larvae.
Biography
Chisato Kataoka is a PhD candidate of Toyo University and received award of Research Fellow of Japan Society for the Promotion of Science. Her research
includes Nanotoxicology, Ecotoxicology, Immunotoxicology and intestinal bacteria using medaka fish (Oryzias latipes). She has been working on fish toxicology of
nanomaterials including silver nano-colloid and carbon nanotubes since 2013. She has published four papers and one review paper. Through her intensive studies,
recently she has received best student presentation awards in international meetings (Aquatic Animals Models of Human Diseases Conference, and Pollutant
Responses in Marine Organisms). Her affiliated academic societies are Society of Environmental Toxicology and Chemistry(SETAC), and The Japanese Society
of Environmental Toxicology (JSET).
chisato.kataoka1@gmail.comChisato Kataoka et al., J Earth Sci Clim Change 2017, 8:10(Suppl)
DOI: 10.4172/2157-7617-C1-037