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conferenceseries
.com
Volume 9
Journal of Nutrition & Food Sciences
ISSN: 2155-9600
Clinical Nutrition 2019
March 04-06, 2019
March 04-06, 2019 | Barcelona, Spain
24
th
International Conference on
Clinical Nutrition
The antibacterial effect of probiotic Nis-Lact-Bif on E. coli and Campylobacter jejuni
Hamidreza Ebrahimi Nezhad
Baqiyatallah University of Medical Science, Iran
Background
: Probiotics are live microorganisms acting through varied mechanisms affecting the density of the
commensal microbiota against pathogens. Nowadays, because of the problems associated with antibiotic use,
probiotic strains provide a new and better option for treatment of infectious diseases like diarrhea. The aim of this
study was to investigate the antibacterial synergism of
Lactobacillus
spp.,
Bifidobacterium
spp. and Escherichia coli
strain Nissle 1917 (ECN) on clinical sample of diarrheagenic
E. coli
and
Campylobacter jejuni
.
Methods
: A paper disk diffusion technique used to evaluate the antibacterial activity. Sterile 6 mm paper disks were
saturated with probiotic suspensions made by settling probiotic medications into distilled water. Three kinds of
disks were prepared. One disk for
Lactobacillus
spp. and
Bifidobacterium
spp., another disk for ECN and third disk
were made by mixing probiotics. Clinical sample of diarrheagenic
E. coli
and
Campylobacter jejuni
were cultivated
on separated Muller Hinton agars and disks were placed on the inoculated Muller Hinton agars. The plates were
incubated with a microaerophilic gas pack inside an anaerobiosis jar, for 24 h at 37°C.
Results
: The Zone of Inhibition (ZOI) of bacterial growth was measured. All pathogenic microorganisms showed
sensitivity to the probiotic disks. The combined disk had a better effect against pathogens.
Conclusion
: A considerable synergistic effect of probiotic strains was observed and it means that combined strains
can be more efficient against intestinal pathogens.
hamidrezaebi20@gmail.comJ Nutr Food Sci 2019, Volume 9
DOI: 10.4172/2155-9600-C2-093