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Volume 10 Journal of Cancer Science & Therapy
ISSN: 1948-5956 Euro Cancer 2018
July 23-25, 2018
July 23-25, 2018 | Rome, Italy
29th Euro-Global Summit on
Cancer Therapy & Radiation Oncology
In vivo quantitation of circulating tumor cells with high-speed confocal microscopy in mouse tumor
model
Howon Seo
Korea Advanced Institute of Science and Technology, South Korea
The circulating tumor cells (CTCs) have been considered as a seed for cancer metastasis and the level of CTCs in metastatic
cancer patients has been considered as a valuable indicator for predicting the grade of cancer metastasis, efficacy evaluation
of anti-cancer therapy, and potential early diagnosis of cancer recurrence. Currently, ex vivo isolation of CTCs based from
a peripheral blood sample has been a major strategy for the quantitation of CTCs. However, accurate quantitation of rare
CTCs, as few as 1~2 cells per ml of blood sample in patients with metastatic cancer, is technically challenging and suffers
extremely low sensitivity. These limitations can be overcome by using intravital flow cytometry which provides direct detection
and quantitation of circulating cells in blood flow. For direct observation of fast-flowing cancer cells in the bloodstream,
a custom-built high-speed video-rate laser-scanning confocal microscope system was implemented. After the intravenous
injection of cancer cells and long circulating reference cells such as red blood cells (RBCs), a dynamically changing number
of circulating cancer cells and RBCs was continuously monitored by real-time imaging. By extracting the calibration factor
from hemocytometric imaging analyses with intravenously injected RBCs, we could estimate the level of intravenous injected
CTCs in the whole blood of a mouse. To evaluate the degrees of cancer metastases with in vivo CTC-quantitation approach, an
orthotopic tumor mouse model was used. The dynamic change of metastatic CTC-level was observed during a few hours and
the longtitudinal change of metastatic CTC was monitored in a single mouse, in vivo.
Biography
Howon Seo is pursuing an Integrated Master’s and Doctoral Degree program at Korea Advanced Institute of Science and Technology in South Korea. He completed his
Bachelor’s Degree in Biochemistry and has worked in intravital imaging of circulating tumor cells with high-speed confocal microscopy. He is interested in the study of
cancer metastasis and metastatic cancer therapy.
Howon Seo, J Cancer Sci Ther 2018, Volume 10
DOI: 10.4172/1948-5956-C8-144