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Volume 6, Issue 9 (Suppl) Dentistry 2016
ISSN: 2161-1122 Dentistry, an open access journal Euro Dental Congress 2016
October 24-26, 2016
October 24-26, 2016 Rome, Italy
15th Euro Congress on
Dental & Oral Health
Martina Sirotkova, Dentistry 2016, 6:9 (Suppl)
Polymorphisms in genes encoding purinoreceptor, osteoprotegerin and external apical root resorption
in children after orthodontic treatment
Martina Sirotkova
Masaryk University, Czech Republic
Orthodontically induced external apical root resorption (EARR) is a multifactorial inflammatory disease. Genes encoding proteins
which are involved in the modulation of inflammatory response and bone remodeling are considered as the “Candidate” for
EARR. The aim of this study was to analyze possible associations of single nucleotide polymorphisms (SNPs) in the P2RX7 (encoding
purinoreceptor) and TNFRSF11B (encoding osteoprotegerin) genes with EARR in Czech children after orthodontic treatment.
A total of, 99 orthodontically treated patients (69 healthy and 30 with EARR) were enrolled in this case-control study. Genotype
determination of P2RX7+489C/T (rs208294, Tyr155His) and +1068C/T (rs1718119, Thr348Ala), TNFRSF11B -163C/T (rs3102735)
and TNFRSF11B+1181C/G (rs2073618, Lys3Asn) was based on polymerase chain reaction using 5′ nuclease TaqMan® assays.
While no significant differences were observed in allele or genotype frequencies of all four studied SNPs, specific combinations of
variants in P2RX7 may be associated with lower/higher risk of EARR development (P<0.05). In addition, the length of treatment by
orthodontic appliances positively correlated with the presence of EARR (P<0.05). Although the effect of P2RX7 SNPs themselves to
the development of EARR was not confirmed in the Czech population, haplotype analysis suggests that variability in the P2RX7 gene
as well as the length of treatment may be important factors contributing to the etiopathogenesis of post-orthodontic EARR.
Biography
Martina Sirotkova, have just finished the fourth year of dental medicine at the Faculty of Medicine, Masaryk University in Brno - one of the top ranked universities
in the Czech Republic. Next academic year they are going to participate in the Erasmus program and study at University Tor Vergata in Rome, Italy. They are
determined and motivated students, and they are very keen on attending various lectures, workshops as well as dedicating their free time to research work.