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conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Gastro Congress 2018
August 20-21, 2018
August 20-21, 2018 | Rome, Italy
13
th
Euro-Global
Gastroenterology Conference
Prevelance of occult HBV in chronic hepatitis C and cryptogenic hepatitis patients
Bülent Çakal
Istanbul University, Turkey
O
ccult hepatitis B Virus (HBV) infection (OBI) is considered as the possible a phase of the HBV natural history but it
remains unclear the molecular mechanisms and clinical impact and epidemiology aspect of OBİ. We investigated the
prevalence of OBI and its clinical impact among patients with Hepatitis C virus (HCV) infection and with cryptogenic hepatitis.
This study protocol was approved by the ethics committee of İstanbul University- School of Medicine (No: 2015/1519). This
prospective cohort study included a total of 60 HBsAg-negative patients (27 patients with chronic HCV and 33 patients with
cryptogenic hepatitis) were enrolled in Department of Gastroenterology, Istanbul Faculty of Medicine. Liver tissue samples
had been obtained by percutaneous needle liver biopsy and immediately frozen and stored at -80°C. Total nucleic acids were
extracted from frozen liver biopsies using QIAamp DNA Mini Kit (Qiagen) according to the manufacturer’s instructions. OBI
was defined as HBV DNA positivity in two or more different viral genomic regions by nested polymerase chain reaction PCR
using 4 sets of primers in preS-S (S), precore-core (C), Pol, and X viral regions of the HBV genome. Plasmid HBV DNA 4.1 kb
and liver biopsy samples obtained from patients with chronic HBV infection (positive control) were used. Statistical analyses
were evaluated using Mann–Whitney U, Chi-square test and Kruskal Wallis tests. The baseline characteristics of patients are
presented in figure 1. The prevalence of OBI was 25.9% (7/26) with 27.3% (9/33), 26.7% (16/60) in patients anti-HCV (+),
cryptogenic hepatitis, and totaly respectively. There wasn’t significant differences for prevelance of OBİ between patients with
Chronic HCV infection and cryptogenic hepatitis (P=0.907). Patients with anti-HCV (+), OBİ (+) were older compared with
patients anti-HCV (+), OBİ (-), (P: 0.033). As it is expected that cryptogenic hepatitis patients had higher serum alkaline
phosphatase and gamma-glutamyltransferase level. Clinical signifance and role of OBI in patients with chronic HCV infection
is controversial. According to first results of the study, prevelance of OBİ is correlated with endemicity of Hepatitis B infection
moreover OBİ can be associated with liver injury rather than chronic HCV infection. Therefore, it appears that host factors
rather than viral factors are more responsible for OBI.
bulentcakal@yahoo.comJ Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C5-077