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Volume 8 Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X Gastro Congress 2018
August 20-21, 2018
August 20-21, 2018 | Rome, Italy
13th Euro-Global
Gastroenterology Conference
Ceramide as a mark of severity and activity of diabetes mellitus type 2 and nonalcoholic fatty liver
disease
Leonid Pavlovskyi
National Medical University, Ukraine
Introduction: Sphingolipids are biological active components of all cellmembrane.They play a great role in cell interconnections
and take part in such process as a proliferation, maturation and cell apoptosis. Ceramide deserve special an attention, because
they could be precursors of apoptosis and lead to development of diabetes mellitus type 2 (DM type 2) and nonalcoholic fatty
liver disease (NAFLD).
Objectives: The objective of this study is to investigate dependence between ceramide levels in plasma, level of insulin and
HOMA (homeostatic model assessment) indices in patients with DM type 2 and activity of AST(aspartate transaminase), ALT
(alanine transaminase) in patients with NAFLD.
Materials & Methods: We performed researches in three groups. The total number of patients was 60 (n=60). Two of them
consisted of patients with DM type 2 and NAFLD (n=20, n=20 respectively) and the third one was healthy persons. The level
of insulin, HOMA indices and activity of AST and ALT were assessed by using common biochemical blood analyses. Plasma
ceramides (C14:0, C16:0, C18:0, C18:1, C20:0, C24:0 and C24:1) were quantified using electrospray ionization tandem mass
spectrometry after separation with HPLC.
Results: As result of researches, we got data that show high levels of ceramide in the first and second group comparing with
healthy persons. This result is tightly correlated with the high level of insulin, HOMA indices and activity of AST and ALT in
patients with DM type 2 and NAFLD respectively.
Conclusions: During our researches, we found out dependence between high ceramide levels with severity of DM type 2 and
activity rate of NAFLD. So, further investigation of ceramide is needed for taking them as parameters for prognosis DM type
2 severity or as development NAFLD complications such as nonalcoholic steatohepatitis.
Biography
Leonid Pavlovskyi, Doctor graduated from National Medical University. She is currently working in Gastroenterology Department in one of the central hospital. She
also works as an Assistant of Internal Medicine Department of National Medical University.
Leonid Pavlovskyi, J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C5-077