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Volume 8 Gynecology & Obstetrics Gynecology Congress 2018
August 22-23, 2018
August 22-23, 2018 Tokyo, Japan
5 th Asia Pacific
Gynecology and Obstetrics Congress
Romanov Andrey Yurievich et al., Gynecol Obstet 2018, Volume 8
DOI: 10.4172/2161-0932-C3-030
Human blastocyst hatching success in the IVF cycles: Cell and genetic predictors
Romanov Andrey Yurievich, Makarova Nataliya Petrovna, Burmenskaya Olga Vladimirovna and Dolgushina Nataliya Vitalievna
National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Healthcare of the Russian Federation, Russia
Background: According to in vitro experiments, up to 75%of morphologically normal human blastocysts cannot spontaneously
leave the Zona Pellucida (ZP), which can lead to implantation failure.
Aim: To identify predictors of human blastocyst hatching success in assisted reproduction programs.
Materials & Methods: This prospective case-control study included 83 blastocysts; 36 blastocysts (43.4%) in group of
spontaneous hatching and 47 blastocysts (56.6%) in control group. Hatching success assessment was performed after 144-146
hours post fertilization. The expression of cathepsin V (CTSV), GATA binding protein 3 (GATA3) and human Chorionic
Gonadotropin Beta (CGB) genes were detected by qRT-PCR (DNA Technology, Russia).
Results & Discussion: The efficacy of spontaneous hatching of human blastocysts is determined not by the quality of the ZP,
but by the quality of the blastocysts themselves. The rate of blastocysts with the thickening or other defects of the ZP was similar
in the two groups. High-quality embryos have enough adaptation possibilities for the timely release both from the normal and
the thickened ZP. The quality of blastocysts on the fifth day of culture by Gardner classification was higher in the group of
spontaneous hatching. Expression of CTSV, GATA3 and CGB genes was higher in blastocysts in spontaneous hatching group,
which was associated with high ICM and TE quality. This indicates that hatching is a special stage of blastocyst development,
characterized by chronological and chrono-genetic determinism. This may be a mechanism that prevents the implantation of
a defective embryo with retarded development or other development disorders. The expression of CTSV, GATA3 and CGB
genes is lower in low-quality blastocysts, which does not allow them to commit spontaneous hatching and to implant into the
endometrium.
Biography
Romanov Andrey Yurievich has obtained\his Doctor of Medicine from the Faculty of Fundamental Medicine, Lomonosov Moscow State University, Russia.
From September 2012 to October 2015 he has been working as an Assistant Researcher in the Department of Biochemistry and Molecular Medicine, Faculty
of Fundamental Medicine, Lomonosov Moscow State University. Currently he is pursuing his Residency in National Medical Research Center for Obstetrics,
Gynecology and Perinatology Ministry of Healthcare of the Russian Federation. He is constantly working on developing his practical skills and also performs
research on noninvasive methods of improvement effectiveness of assisted reproduction technologies. He regularly participates in scientific meetings on obstetrics,
gynecology, perinatology and assisted reproduction technologies.