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Journal of Infectious Diseases and Therapy ISSN: 2332-0877 | Volume: 6
Infectious Diseases
4
th
Annual Congress on
Neglected Tropical & Infectious Diseases
5
th
International Conference on
August 29-30, 2018 | Boston, USA
&
Vaccinomics approach for designing potential peptide vaccine by targeting pyruvate kinase of
Madurella
mycetomatis
Aya Yusri A Manofali
1,7
, Ismail M A I
2,7
, Reem E Talha
1,7
, Zahra A Neel
1,7
, Ali A Ali Elamin
1,7
, Alghzali Altayeb M Abdalla
3,7
, Alaa I Mohamed
4,7
, Al Khansa'a M
Othman
4,7
, Dalia A M Hamid
1,7
, Sanaa Bashir
5,7
, Mohammed Shihabeldin
6,7
and
Mohammed A. Hassan
7
1
Omdurman Islamic University, Sudan
2
University of Khartoum, Sudan
3
University of Medical Science and Technology, Sudan
4
Omdurman Islamic University, Sudan
5
University of Khartoum, Sudan
6
Sudan International University, Sudan
7
Africa City of Technology, Sudan
Background:
Mycetoma is one of the neglected tropical diseases that considered as a public health problem with socio-economic
impact in several developing countries. It is a chronic progressive destructive suppurative disease can affect any part of the body,
caused by certain fungi (
Eumycetoma
) or bacteria (
Actinomycetoma
). Madurella mycetomatis(
M mycetomatis
) is the predominant
isolated organism causing eumycetoma in Sudan. .There is no effective treatment or a vaccine for it, thus the aim of this study is to
design a peptide-based vaccine against
M.mycetomatis
infection via in silico approaches, using the immunogenic site Pyruvate kinase
(PK).
Material and Methods:
In 26th September 2017 sequence of PK of
M. mycetomatis
protein was retrieved from the National Center
for Biotechnology Information (NCBI). Immunoinformatics tools were used to predict B and T-cell epitopes and to calculate the
population coverage.
Result and discussion:
Two epitopes predicted for b cell (gsypseav, dftkv) as a peptide-based vaccine. for t-cell epitopes, four
epitopes showed high affinity to mhc class i (amaavrsal, yrgvpflf, hlyrgvypf, yrpvcpiim) and high coverage against the whole world
(58.35%, 57.91%, 54.01%, 52.73%) respectively. in mhc class ii, si\x epitopes that interact with the most frequent mhc class ii (fvlstsges,
ivescamaa, lkaensipy, ikwglshai) with high coverage against the whole world (80.93%, 80.02%, 73.12%,70.55%) respectively. moreover,
one shared epitope (lkaensipy) predicted in b-cell, mhc-i, and mhc-ii with high population coverage world combined mhc-i and mhc-
ii (77.92%) and (57.78%) in sudan. also, four shared epitopes (yrgvypflf, lkaensipy, lyrgvypfl, ikwglshai) between mhc-i and mhc-ii
with epitope set 94.62% worldwide and 92.38% in sudan. till now there is no study was done to predict peptide-based vaccine against
mycetoma infection, so this study will provide a strong base for development of vaccine after
in vivo
and
in vitro
studies confirmation
of all this candidate epitopes as effective peptide vaccine.
ali1993alameen@gmail.comJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C3-045