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Journal of Infectious Diseases and Therapy ISSN: 2332-0877 | Volume: 6
Infectious Diseases
4
th
Annual Congress on
Neglected Tropical & Infectious Diseases
5
th
International Conference on
August 29-30, 2018 | Boston, USA
&
Expression profile of toll-like receptors mrnas in latently infected and non-infected school: Age children
and adolescents
Birhan Alemnew
1,2
, Tamirat Abebe
1
, Soren Hoff
3
, Abraham Aseffa
1
, Rawleigh Howe
1
, Liya Wassie
1
1
Addis Ababa University, Ethiopia
2
Woldeya University, Ethiopia
3
Statens Serum Institute, Denmark
Background:
About one-third of the global population is considered to be latently infected with tuberculosis (TB) causing bacteria,
Mycobacterium tuberculosis
. Only 10% progress to active disease, while the majority contain the infection. Latently infected individuals
are considered as reservoirs for continuous new TB infections.
Objective:
The aim of this study is to measure the mean fold change in mRNA gene expression of Toll-like receptors (TLRs) during
latent TB infection as a diagnostic biomarker tool for detection of TB progression.
Methodology:
Quantitative real-time PCR (qRT-PCR) was used to measure the expression of selected TLRs (TLR-1, TLR-2, TLR-4,
TLR-6 and TLR-9) in a total of 64 cDNA samples, retrieved fromAHRI biorepository and collected from 32 tuberculin skin test (TST)
positive and 32 TST negative, using convenient sampling, apparently healthy school children and adolescents, aged between 11 and 20
years. Specific primers and fluorescent labeled probes were used to span exon-intron junctions to prevent amplification of genomic
DNA. Human acidic ribosomal protein (HuPO) was used as an internal control. A comparative CT method was used to describe fold
change in the relative expression of TLR genes. Data were analyzed using Graph-Pad Prism 7.01 for Windows and a p-value of less
than 0.05 was considered statistically significant. This study was approved by the AHRI/ALERT Ethics Review Committee (AAERC),
AAU-CHS IRB and the National Research Ethics Review Committee (NRERC).
Result:
An increased mean fold change in the relative expression of TLR-2 and TLR-6 mRNA was observed in TST positives relative
to TST negatives (p<0.05), whereas a slight fold decrease was observed for TLR-1 gene. A strong positive linear correlation (r=0.7)
was also observed between intra-compartment receptor, TLR-9 and surface receptors, TLR-1, TLR-2, TLR-4, and TLR-6 expression
in latently infected tuberculosis relative to non-infected. A similar comparison was done between different age groups and both sexes;
however, no apparent difference was observed in the fold change expression of TLRs.
Conclusion:
Overall, an increased mean fold change in the mRNA expression of TLRs was observed in latently infected individuals
relative to non-latently infected individuals, possibly showing a role for TLRs during latent tuberculosis infection, thereby maintaining
and continuously stimulating immune responses through TLRs signaling pathways.
birhanalemnew12@gmail.comJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C3-045