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Journal of Infectious Diseases and Therapy ISSN: 2332-0877 | Volume: 6
Infectious Diseases
4
th
Annual Congress on
Neglected Tropical & Infectious Diseases
5
th
International Conference on
August 29-30, 2018 | Boston, USA
&
Macrofilaricidal activity of silver nanoparticle synthesized from a plant andrographis paniculata
Smita Yadav
Banaras Hindu University, India
L
ymphatic filariasis is a neglected tropical disease, causing a major health hazard in the developing world. WHO has ranked the
disease as one of the world’s leading causes of permanent and long-term disability. The currently available anti-filarial drugs
are most effective against microfilaria. Therefore there is urgent need of drug that are macrofilaricidal. Nanoparticles have gained
significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesize AgNPs from a medicinally
important plant Andrographis paniculata. The plant have been reported for their antimicrobial, cytotoxicity, anti-protozoan, anti-
inflammatory, anti-oxidant, and antiparasitic activities. This nanocomposite was characterized by UV-visible spectroscopy, FT-IR,
XRD, SEM, and TEM. Nanocomposite anti-filarial activity was evaluated using motility and viability assay as well as by measuring
ROS generation, antioxidant level, and apoptotic markers. The exposure of the nanocomposite to the worms caused a significant
decrease in motility and viability leading to their death. Down-regulation of the antioxidant enzymes, as well as alteration in Ca2+
signaling, suggested the ER stress-induced mitochondrial-mediated apoptosis. The proteome analysis of treated parasites showed the
marked alteration in the protein expression in comparison to the control. In conclusion, the nanocomposite synthesized using plant
A. paniculata showed strong anti-filarial activity.
yadav.smita7@gmail.comJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C3-045