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conferenceseries
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Volume 7, Issue 1 (Suppl)
J Clin Exp Pathol
ISSN: 2161-0681 JCEP, an open access journal
Pediatric Pathology & Laboratory Medicine 2017
March 15-16, 2017
March 15-16, 2017 London, UK
12
th
International Conference on
Pediatric Pathology & Laboratory Medicine
Unusual HBV mixed genotype infections among hepatitis type B Iraqi patients in Wasit Province, Iraq
Sareaa Maseer Gatya Al-Mayahie
1
, Ali Shakir Kamil Al-Suraifi
1
Ahmed Darwish Jabbar Al-Rubaie
1
and
Naeem Mohammed Mohsen Al-Abedy
2
1
Wasit University, Iraq
2
Alkarama Teaching Hospital, Iraq
H
epatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from
HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as
a minimum 10 genotypes (A to J) and numerous sub-genotypes have been identified. Hepatitis B virus variants may be differing in
their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried
out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province, Iraq using nested PCR protocol. A total of
105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected with viral hepatitis were included in this study. All
the patients’ sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas, 72 (60.5%) and
33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples
for HBV genotypes by nested PCR (second PCR) demonstrated unique results that no single genotype was found and all of these
samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%),
A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was
non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV
genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different
times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all
over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.
sareaamaseer@gmail.comJ Clin Exp Pathol 2017, 7:1 (Suppl)
http://dx.doi.org/10.4172/2161-0681.C1.032