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.com
Volume 8
Journal of Clinical Trials
Pharmacy and Pharmacovigilance 2018
July 16-17, 2018
July 16-17, 2018 Sydney, Australia
Joint Event on
Global Pharmacovigilance and
Advanced Pharmacy
In vitro
immunomodulatory activity of chrysin (5, 7-dihydroxyflavone) isolated from
Indigofera
tinctoria
on macrophage via NF-κB signaling pathway
Ravichandran Ramanibai
*
and Madakkannu Boothapandi
University of Madras, India.
I
n the present investigation, in vitro immunomodulatory effect of natural flavonoid chrysin isolated from Indigofera tinctoria leaves
was evaluated on murine macrophages (RAW 264.7 cells). The chrysin was purified and structure was elucidated by spectroscopic
analysis including UV- visible, FTIR, NMR (13C, 1H) and GC–MS. The immunomodulatory activity of different concentrations of
chrysin (25, 50, 75 and 100 μg/mL) was analyzed on normal and LPS(10 µg/mL) stimulated macrophage with various assays including
cell proliferation, nitric oxide (NO) production, phagocytosis, ROS generation, gene expression and NF-κB nuclear translocation. The
results revealed that, chrysin was significantly increased the proliferation of macrophages as well as phagocytic function of macrophages
in dose dependently. Moreover, the preliminary and essential features of phagocytosis such as superoxide anions production, lysosomal
and pinocytic activity of macrophages was significantly enhanced upon chrysin treatment. On the other hand chrysin significantly
debited the ROS generation, NO production and increased the arginase activity on LPS stimulated macrophage. The expression of
pro-inflammatory cytokine such as TNF-α & IL-6 and pro-inflammatory mediators such as iNOS & COX-2 and the expression also
dose dependently decreased by chrysin in LPS treated macrophages. Moreover, the chrysin treatment was greatly reduced the NF-κB
activation and subsequently decreased the NF-κB nuclear translocation. Collectively, the present findings suggested that a natural
flavonoid chrysin can increased the innate immune response by enhancing macrophages functions through NF-κB signaling pathway
and explored the strong immunomodulatory potential.
rramani8@hotmail.comJ Clin Trials 2018, Volume 8
DOI: 10.4172/2167-0870-C2-026