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Volume 8, Issue 4 (Suppl) J Vet Sci Technol, an open access journal
ISSN: 2157-7579 Veterinary 2017
September 04-05, 2017
September 04-05, 2017 | Paris, France
7th International Veterinary Congress
Presence of Salmonella spp. in ground beef and cattle meatball
Belgin Sırıken1, Ceren Yavuz2 and Tuba Yıldırım2
1Ondokuz Mayis University, Turkey
2Amasya University, Turkey
Statement of the Problem: Nowadays, millions of people have died because of the foodborne diseases. Cattle origin meats have also
important role in human salmonellosis. The aim of this study was to determine Salmonella spp. in ground beef and raw meatball
(cattle origin) samples consumed in Amasya province, Turkey.
Methodology & Theoretical Orientation: In the study, a total 100 samples (50 ground beef and 50 meatball samples) randomly
collected from supermarkets and butchers in Amasya province were analyzed. Two enrichment step classic culture technique
was applied for the microbiologic isolation. For the isolation, Buffered Peptone Water (BPW) was used for pre-enrichment step
and Rappaport Vassiliadis Broth (RV-Broth) was applied for selective enrichment step and Xylose Lysine Tergitol 4 (XLT4 with
supplement) was used for the selective agar. For the confirmation of the isolates in molecular levels, single target PCR assay was used.
For this purpose, invA and oriC genes were determined in the isolates.
Findings: Salmonella spp. were determined in 6 (6%) of samples. Distribution of 6 samples; 4 (n=50, 8%) of 6 was determined in
ground beef samples and 2 (n=50, 4%) of 6 was determined in meatball samples.
Conclusion & Significance: Global incidence of Salmonella spp. infections in humans has shown a significant increase. There have
been various Salmonella isolation ratio of ground beef and meatball samples reported from different part of the world, and the results
are changing from 0.0% to 26.7%. In the Turkey, Salmonella spp. contamination ratio is changing from 0.0% to 18.0%. Our study
results are between these ratios. To our knowledge, there has not been a study on this matter. As a result, ground beef and meatballs
were contaminated with the most widespread foodborne bacteria, Salmonella spp. These kinds of samples may be a potential vehicle of
transmission of Salmonella spp. to humans. Therefore, it is determined that Salmonella could be threat to public health via consumed
ground beef or meatball samples.
Biography
Belgin Sırıken is an expert in Food Microbiology, Safety and Chemical Properties of Particularly Animal Origin Foods. She has completed her PhD at Ankara
University, and now she is working as Prof. Dr. at Ondokuz Mayis University, Samsun, Turkey. Her focus is on Molecular Food Microbiology.
Belgin Sırıken et al., J Vet Sci Technol 2017, 8:4(Suppl)
DOI: 10.4172/2157-7579-C1-024