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.com
Volume 8, Issue 4 (Suppl)
J Vet Sci Technol, an open access journal
ISSN: 2157-7579
Veterinary 2017
September 04-05, 2017
September 04-05, 2017 | Paris, France
7
th
International Veterinary Congress
Investigation of probiotic properties of chicken originated
Enterococcus faecium
and
Lactobacillus
species*
Alper Ciftci
and
Yagmur Kocak
Ondokuz Mayis University, Turkey
Statement of the Problem
: In this study, the isolation of
Lactobacillus
spp. and
Enterococcus faecium
and investigation of usage
potential of isolated bacteria as probiotic were aimed.
Methodology & Theoretical Orientation
: In a study, 50 chicken intestines were investigated which were taken from a commercial
chicken slaughter house in Samsun region. The samples were taken from intestine mucosa and inoculated to the selective mediums for
the isolation of
Lactobacillus
spp. and
E. faecium
. Suspected colonies were identified by PCR. The isolated bacteria were investigated
for bile (0.5-1%) and pH (3-5) resistance by bile and pH tolerance tests, respectively. The hydrophobicity’s of the isolates were tested
by using 0.03% Congo Red Agar. The antibiotic resistances of the isolates were determined by Agar Gel Disc Diffusion Test with using
9 antibiotic discs. The Radial Diffusion Method was used for determining the antagonistic effects of the isolates against
Escherichia
coli
.
Findings
: Twenty E.
faecium
, 21
Lactobacillus
spp. (9 of them were L.
acidophilus
) isolated from samples. All the isolates were found
as resistant to tested bile and pH conditions. All the isolates were hydrophobic, but none of them had an antagonistic effect against
Escherichia coli
. Eight of E.
faecium
isolates were found as resistant to 8 antibiotics. One
Lactobacillus
spp. (other than L.
acidophilus
)
and 1 L.
acidophilus
isolates were resistant to 5 and 7 antibiotics, respectively. These isolates were evaluated as multi-antibiotic resistant
strains.
Conclusion & Significance
: In conclusion, we evaluated that all the isolates were hydrophobic, resistant to bile and low pH conditions;
but none of them had an antagonistic effect against
Escherichia coli
in tested conditions. These results indicated that the multi-
antibiotic resistant strains of
E. faecium, L. acidophilus
and
Lactobacillus
spp. isolates had a potential of using as a probiotic and
further in vivo studies had to be essential for these strains.
*Thiswork is supportedbyScientificResearchProjectsCommissionofOndokuzMayisUniversity (ProjectNo: PYO.VET11904.16.016).
Biography
Alper Ciftci is an expert in Molecular Microbiology and Vaccine Development. He has completed his PhD at Ankara University, and now he works as Associate
Professor at Ondokuz Mayis University, Samsun, Turkey. He focuses on working development and validation of commercial products such as vaccine and
diagnostic kits.
aciftci@omu.edu.trAlper Ciftci et al., J Vet Sci Technol 2017, 8:4(Suppl)
DOI: 10.4172/2157-7579-C1-024