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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Enantiomer separations of amino acids and derivatives on immobilized polysaccharide phases with
extended range of solvents using supercritical fluid chromatography
Tony Q Yan, Frank Riley, Laurence Philippe, Mark Hardink, Jared Van Haitsma and John Salisbury
Pfizer Inc., USA
This presentation discusses immobilized polysaccharide stationary phases with extended range of solvents for enantiomer
resolution of select amino acids and derivatives using Supercritical Fluid Chromatography (SFC) and High Performance
Liquid Chromatography (HPLC). Baseline resolution is achieved for selected amino acids and derivatives using this approach.
The combination of an extended solvent (normally an aprotic solvent) in the presence of methanol and a suitable additive such
as water, acid and base are critical for retention and resolution of amino acid pairs on immobilized columns. The amino acid
separations are also achieved on the coated polysaccharide phases with alcohol based solvents in the presence of acid and base.
In addition, the use of cyclofructan and polysaccharide phases for amino acids and small peptides separations along with the
use of reversed phase chiral stationary phase for very polar compounds, acids and diacids, are also discussed.
Biography
Tony Q Yan is currently working for Pfizer, Inc. (Groton, CT, USA) in the field of impurity isolation for structure elucidation in the Department of Pharmaceutical
Science. He has been working in pharmaceutical research and development in the area of chiral and achiral purifications, and impurity isolation for over 20 years
since he graduated from the Department of Chemistry in University of Missouri in Rolla with PhD degree in 1995.
Tony Q Yan et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)