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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Removal of lipid interferences using zirconia-based Solid Phase Extraction (SPE) andQuEChERS sorbents
Jennifer E Claus¹, Katherine Stenerson¹, Olga Shimelis¹, Klaus Buckendahl², Candace Price¹ and Michael Ye¹
¹MilliporeSigma, USA
²Sigma-Aldrich Chemie GmbH, Germany
Although lipids are essential to life processes, they pose an obstacle in both bioanalysis and food analysis. In addition to
producing inaccurate detection limits and contaminating chromatographic systems, interfering lipids can ultimately lead
to the shortened lifespan of columns and instruments. Traditional solid phase extraction (SPE) cleanup techniques often provide
insufficient lipid matrix removal. Therefore, the use of zirconia-based sorbents has been developed for selective lipid removal,
and consequently, better analyte determination. In addition to hydrophobic interactions, zirconia-based sorbents utilize Lewis
acid/base interactions to selectively retain undesirable lipid interferences. In SPE and/or dispersive SPE (QuEChERS) formats,
these zirconia sorbents may be combined with traditional phases like C18 to further improve inference removal. Compared to
traditional cleanup sorbents, these innovative sorbents have been shown to remove more lipid matrix interferences, including
di, tri-, monoglycerides and phospholipids. A comparison of zirconia-based sorbents to traditional cleanup sorbents for
lipid removal from various food and biological matrices will be demonstrated. Background removal, analyte recovery, and
reproducibility of the different cleanup techniques will be compared in this presentation.
Biography
Jennifer E Claus has graduated from Lycoming College in 2001 with a Bachelor of Science in Chemistry and Biology. She has spent six years working at Merck
& Co. in Rahway, NJ as a Medicinal Chemist. While at Merck, she attended Rutgers University and completed her Master of Science in Chemistry. She has been
with MilliporeSigma for the past nine years, initially working as a Chiral Applications Chemist for the first four years of her MilliporeSigma career. She has been in
her current marketing position as a Product Manager for Sample Preparation, for the past four years.
Jennifer E Claus et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)