chromatography-2016_scientifictracks-abstracts

25 / 32

Notes:
Page 53
Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Raman spectroscopy based method for the evaluation of compositional consistency of nanofibrous layers
Adéla Kotzianová1,2, Jiří Řebíček3, Ondřej Žídek1, Marek Pokorný3, Jan Hrbáč2 and Vladimír Velebný1,3
1Contipro Pharma a.s., Czech Republic
2Masaryk University, Czech Republic
3Contipro Biotech s.r.o., Czech Republic
Electrospinning is currently a very popular method used across a number of industries. Electrospinning enables the
production of nanofibrous layers of various structures and compositions.The production of amulti-component nanofibrous
layer may result in an uneven distribution of the individual components throughout the layer. Confocal Raman spectroscopy
combined with statistical methods allows these layers to be analyzed by determining their chemical composition and thus
provides feedback for the spinning process. This paper presents a method which combines Raman spectroscopy analysis and
its subsequent evaluation with singular value decomposition (SVD). Automated measurement of Raman spectra makes it
possible to gather extensive spectral data from a particular area selected on a sample; the spectra are measured from a specific
volume and not from individual fibers. Samples require no preparation for the analysis and the non-destructive nature of
Raman spectroscopy ensures their reusability. When spectra of the individual component materials are included for reference,
the subsequent SVD analysis of the spectral data makes it possible to determine the chemical composition of the measured
areas, thus providing the content percentages of the individual components, which can be displayed either in the form of a
scattered plot or a Raman map.
Biography
Adéla Kotzianová graduated from the Czech Technical University in Prague in 2012, where she earned her Master’s degree in Instruments and Methods for
Biomedicine. Currently, she is finishing her Doctoral studies in Physical Chemistry at the Masaryk University in Brno. Since 2012, she has been working as
a researcher in the Department of Nanotechnology Device Development of the Contipro Holding. She has been specializing in spectroscopic methods since
completion of her studies. In recent years, her work was focused especially on the preparation and analysis of nanofibrous materials. She has published 7 papers
in reputed journals and presented her work on international conferences.
Adéla Kotzianová et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)