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Volume 6, Issue 9 (Suppl) Dentistry 2016
ISSN: 2161-1122 Dentistry, an open access journal Euro Dental Congress 2016
October 24-26, 2016
October 24-26, 2016 Rome, Italy
15th Euro Congress on
Dental & Oral Health
Hyunjung Kim, Dentistry 2016, 6:9 (Suppl)
Improvement of mechanical properties of CPC and MTAby Elastin-Like-Polypeptide
Hyunjung Kim
Kyung Hee University School of Dentistry, Seoul, Korea
The aim of this study is to develop a new elastin-like polypeptide (ELP) supplemented dental repairing cement which shows
improved mechanical and handling properties. ELP genes either with or without octaglutamic acid termination were genetically
engineered: V125 and V125-E8. Pure ELPs were gathered through a series of protein synthesis process using E.coli through gene
transformation, expression, protein purification. 10 wt% ELP solutions were prepared and mixed with mineral trioxide aggregate
(MTA) and calcium phosphate cement (CPC) to have liquid to powder ratio from 0.2 to 0.7. Mechanical property test and washout
test were performed to validate the availability of ELP-MTA mixtures as improved dental repairing cement. In compressive tests,
we prepared from 0.2 to 0.7 ‘liquid : powder’ ratio samples: two powder of CPC and MTA; three liquids of DW, V125, V125E8.
Mixed cements as given ratio, were loaded at mold and set in 37°c incubator for 4 days as column shape. Each samples were tested
in universal testing maching AGS-X (SHIMADZU, Japan). Anti-washout test was performed with the mixed cement of 0.5 liquid/
powder ratio. Each mixed cements soaked with 370HEPES solutions on petri dish were observed at 5 minite, 1 hour and 24 hour. After
drying in 37℃ incubator for 48 hours, the loss weight of cements were also measured. The ELP supplemented MTA and CPC showed
a significantly enhanced compressive strength and anti-washout property. Incorporation of specific ELP enhanced the mechanical
strength and handling property of MTA and CPC. This preliminary test indicates that ELP can be used to develop the inorganic dental
repairing cement to have improved properties.
Acknowledgement : This study was supported by a grant of the Korea Health Technology R&D Project through the Korea Health
Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C1817).
Biography
Hyunjung Kim, DMD
Hyunjung Kim has completed her master degree at 2014 from Kyung Hee University and is pursuing PhD course in same university and undergo the residency
course of conservative dentistry in Kyung Hee University Dental Hospital.
Sun-Young Kim, PhD, DDS
Associate Professor in Dept. of Conservative Dentistry, School of Dentistry, Kyung Hee University
Chair in Dept. of Conservative Dentistry, Kyung Hee Universtity Dental Hospital