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Volume 6, Issue 9 (Suppl) Dentistry 2016
ISSN: 2161-1122 Dentistry, an open access journal Euro Dental Congress 2016
October 24-26, 2016
October 24-26, 2016 Rome, Italy
15th Euro Congress on
Dental & Oral Health
Suk Won Lee, Dentistry 2016, 6:9 (Suppl)
Determination of osteoblastic differentiation and osteogenic transcription factor expression on
fibronectin or bone sialoprotein II-immobilized microgrooved titanium substrata
Suk Won Lee
Kyung Hee University, South Korea
Here, we aimed to determine the effect of fibronectin (FN) or bone sialoprotein Ⅱ (BSP2)-conjugated microgrooved titanium (Ti)
substrata on osteoblastic differentiation and time-dependent osteogenic transcription factor expression in human bone marrow
mesenchymal stem cells (MSCs). 60 µm wide and 10 µm deep microgrooves were fabricated using photolithography and subsequent
acid etching to generate a microgrooved Ti surface with acid-etched roughness (E60/10). Both smooth and acid-etched Ti were
used as controls (NE0 and E0). Human serum FN and human BSP2 were immobilized on the fabricated Ti surfaces by silanization
using 3-aminopropyltriethoxysilane (NE0FN, E0FN, E60/10FN, NE0BSP2, E0BSP2, and E60/10BSP2). Alkaline phosphatase (ALP)
activity and osteoblastic differentiation of MSCs were determined using ALP activity assay and extracellular calcium deposition assay,
respectively. Time-dependent expression of various osteogenic transcription factors including ATF4, FRA1, RUNX2 and Osterix
were analyzed. As a result, both FN- and BSP2-immobilized microgrooved Ti significantly enhanced the osteoblastic differentiation
and the time-dependent expression of various osteogenic transcription factors. Specifically, the two-way ANOVA result of ATF4,
FRA1, RUNX2, and Osterix expression presents a synergistic promotion effect of microgrooves and matrix protein immobiliztion
on the osteoblastic differentiation of MSCs. Taken together, FN- and BSP2-immobilized microgrooved Ti can act as an effective
biomaterial surface for promoting osteogenicity.
Biography
Suk Won Lee has completed his PhD and Post-doctoral fellowship from Yonsei University College of Dentistry. He is currently an Associate Professor of Kyung
Hee University College of Dentistry. He has published more than 25 papers in reputed journals in the fields of Biomaterials, Biomedical Engineering and Oral
Implantology. He has been serving as Director, Education Delegate and Editorial Board Member of repute.