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conferenceseries
.com
Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
MicroRNA expression profiling in placenta and maternal plasma in early pregnancy loss
Mohammad Kazem Hosseini
1
, Tuba Gunel
1
, Ece Gumusoglu
1
, Ali Benian
1
and
Kilic Aydinli
2
1
Istanbul University, Turkey
2
Medicus Health Center, Turkey
E
arly pregnancy loss (EPL), is determined as the unintentional expulsion of an embryo or fetus prior to the 12th week of
gestation. EPL frequency is ~15% in pregnancies. Fetal development and growth is associated with placental function
and vessel development; therefore, the placental genome would represent a useful EPL model for epigenetic and genomic
studies. An important factor of placental development and function is epigenetic regulation of gene expression. MicroRNAs
(miRNAs) are the primary epigenetic regulators which have an important role in placental development and function. In the
present study, maternal plasma and villous tissue were collected from 16 EPL cases during 6
th
‑8
th
gestational weeks (GWs)
and 8 abortions (control group) in 6
th
‑ 8
th
GWs. Detection of the differences in miRNA expression was performed using
microarrays and dysregulated miRNAs were validated by RT‑qPCR. miRNA microarray findings revealed that four miRNAs,
including hsa‑miRNA (miR‑125a‑3p, hsa‑ miR‑3663‑3p, hsa‑miR‑423‑5p and hsa‑miR‑575 were upregulated in tissue
samples. In maternal plasma, two miRNAs (hsa‑let‑7c, hsa‑miR‑122) were upregulated and one miRNA (hsa‑miR‑135a) was
downregulated. A total of 6 out of 7 dysregulated miRNAs were validated using RT‑qPCR. The aim this study was to detect
dysregulated miRNAs in maternal plasma and villous cells and identify the target genes of dysregulated miRNAs and their
associated pathways. The target gene analyses have revealed that the affected genes are primarily associated with cell migration,
proliferation, implantation, adhesion, angiogenesis and differentiation and all are involved with EPL pathogenesis. Therefore,
the present study may contribute to the understanding of the molecular mechanisms which lead to EPL.
mohammad_h556@yahoo.comJ Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028