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Volume 12 Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6th International Conference on
Genomics and Molecular Biology
10th International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Regenerative competence in root explants of Rhynchostylis gigantea, an endangered species: In vitro study
Vishal Sharma
Post Graduate Government College for Girls - Panjab University, India
Orchids constitute one of the largest and diverse family of angiosperms families with 30,000-35,000 species in 600-800
genera, still in an evolutionary flux. They have out-smarted and out-numbered their counterparts due to their long-
lasting flowers of myraid shapes, sizes and colors. Their latter utility accounts for a highly lucrative trade in floriculture. Tissue
culture technique has been exploited as means of ex situ conservation, particularly in outbreeders like orchids which generate
a great deal of heterozygosity in the progenies Beechey (1970) suggested possibility of using aerial roots in micro-propagating
orchids. The utility of roots as explant source is being increasingly realized due to their easy availability, low oxidation rate &
ease with which they can be planted. Keeping this in view, presently we report the pioneer attempt to use root explants from
in vivo grown fox tail orchid, R. gigantea (Lindl.) Ridl, a native of Thailand. R. gigantea exhibit free fertility within and beyond
the taxonomic limits and has been used as breeding material for raising floriculturally significant hybrids. Besides being victim
of its own beauty & utility R. gigantea is progressively losing its natural habitat and is getting rarer with every passage of
time and figures prominently in Appendix II of the Convention on International Trade in Endangered species of Wild Fauna
and Flora (CITES,2012,2017). The neo-formations in the in vivo root explants of Rhynchostylis gigantea depend upon their
location, maturity level and chemical regime. The regeneration is affected by polarity showing basipetal gradient. The distal
ones with intact tips with well-developed root caps showed an extended growth with sub-apical formation of globular structure
whereas, the proximal explants responded to the presence of cytokinin (Kn) medium according to Mitra et al. (1976) The effect
of cytokinins was accentuated in the additional presence of NAA and the higher organogenetic responses are observed in
explants when BAP, Kn was used in dose double than that of NAA. The regenerated plantlets were acclimatized & transferred
to pots filled with moss, pine bark, brick &charcoal pieces (2:4:1:1) with 90% survival. In conclusion, the results clearly indicate
that the root segments as a reliable method of clonal propagation bereft of somaclonal as the root explants is an effective
alternative to shoot meristem for micro-propagation due to their easy availability and does not require the sacrifice of mother
plant and provide exciting opportunities to raise large numbers of true-to-type plantlets.
J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028