influenza-2016_scientifictracks-abstracts

Volume 4, Issue 6(Suppl)

J Infect Dis Ther

ISSN: 2332-0877 JIDT, an open access journal

Page 45

Notes:

Influenza 2016

September 12-13, 2016

conferenceseries

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Influenza

September 12-13, 2016 Berlin, Germany

International Conference on

Study on the circulation of influenza A virus in swine populations in Kazakhstan

Nailya Klivleyeva, Glebova T I, Lukmanova G V, Shamenova M G, Saktaganov N T, Ongarbaeva N S

and

Kalkozhaeva M K

Republican State Enterprise on the Right of Economic Management “Institute of Microbiology and Virology” CS MES, Republic of Kazakhstan

mergence of influenza virus A (H1N1)pdm in humans which is a complex reassortant of the swine-origin genotypes, emphasized

the importance of worldwide surveillance for influenza virus in swine. 1293 biosamples (893 nasopharyngeal swabs and 400

blood serums) have been collected from swine in the small and large pig farms of the Almaty, Aktobe, Karaganda, Kostanaу, Pavlodar

and North-Kazakhstan oblasts in 2014-2015. Primary screening of 893 biosamples (nasopharyngeal swabs), carried out in RT-PCR

using AmliSens PCR test system produced by the Central Research Institute for Epidemiology (Moscow) showed the presence of

genetic material of the influenza virus in 171 samples (19.15% of the total number of examined samples). Influenza A/H1 virus RNA

was detected in 111 samples (12.43%), A/H3 virus RNA in 10 samples (1.12%). These data indicate circulating influenza virus of

mixed etiology in the swine population with a predominance of influenza А/Н1 virus. As a result of primary infection of chicken

embryos with 893 biosamples collected from swine in various regions of Kazakhstan, 22 infectious agents were isolated with the

hemagglutination titers of 1:2-1:32 and infectious activity of 3.47-9.45 lgEID50/0.2 ml. Identification in HAI and NAI assays of seven

isolates from the Almaty (06/14 and 10/14), Karaganda (04/14 and 16/14) and Kostanaу (12/14, 23/14 and 24/14) oblasts enabled to

attribute them to influenza A virus with the H1N1 antigenic formula. Serological analysis of 400 blood serums collected from healthy

and sick swine in the pig-breeding farms to detect antibodies against influenza A/H1N1 and A/H3N2 virus was carried out with IEA

and HAI assay. The greatest number of specific antibodies was detected against the influenza virus subtype A/H1N1. Thereby, the

findings confirm the circulation of influenza А/Н1N1 virus in the swine population on the territory of Kazakhstan, which indicates

the need for a permanent virological examination of swine with the aim of the earliest detection of the potential pandemic influenza

virus strain.

Biography

Nailya Klivleyeva is presently working in Institute of Microbiology and Virology” CS MES, Republic of Kazakhstan.

Nailya Klivleyeva et al., J Infect Dis Ther 2016, 4:6(Suppl)