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Volume 4, Issue 6(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 31
Notes:
Influenza 2016
September 12-13, 2016
conferenceseries.com
Influenza
September 12-13, 2016 Berlin, Germany
2nd International Conference on
DIVA tests for avian influenza, which antigen must be chosen?
Farhid Hemmatzadeh, Noor Haliza Hasan, Anne Peaston and Jagoda Ignjatovic
The University of Adelaide, Australia
In last 15 years, numbers of ELISA test were developed to differentiate influenza infected from vaccinated animals (DIVA). In most
of the test the either viral associated infection antigens or heterologous neuraminidase antigens were used to develop DIVA tests.
One of the first attempts was non-structural 1 protein (NS1). The NS1-based ELISA was shown reliable results as a DIVA test in young
chickens but the accuracy of NS1-based DIVA test decreases by the time and numbers of vaccination produces non-specific reactions.
Nucleoprotein (NP) and conserved HA274-288 epitope were the others candidates for DIVA test but these two antigens did not show
any values as DIVA ELISAs. By now the best antigen to develop DIVA-ELISA test is ectodomain of matrix 2 (M2e) protein. Relatively
invariable nature of M2e protein across AIV strains and high level of expression of M2e protein on the surface of infected cells despite
being low in copy number in mature virions are the main properties that make M2e a suitable candidate for DIVA tests. Our studies
on structure of M2e showed the tetramer form of M2e shows higher sensitivity and specificity to discriminate M2e antibodies in sera
of infected birds from vaccinated or non-vaccinated birds.
Biography
Farhid Hemmatzadeh has joined The University of Adelaide as a Senior Lecturer of Virology at the School of Animal & Veterinary Sciences in 2009. Previously, he was
employed by Melbourne University since 2005 and Tehran University as an Associate Professor since 1997. He has over 20 years experience in research and teaching at
the field of animal viral diseases including herpesviruses, pestiviruses, retroviruses, parvoviruses and influenza viruses. He has been involved in research, development
and assessment of diagnostic test for animal viral diseases specially DIVA tests for poultry and large animals.
Farhid Hemmatzadeh et al., J Infect Dis Ther 2016, 4:6(Suppl)