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Volume 4, Issue 6(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 30
Notes:
Influenza 2016
September 12-13, 2016
conferenceseries.com
Influenza
September 12-13, 2016 Berlin, Germany
2nd International Conference on
Electrochemical genosensors based on redox active monolayers: Characterization and applications
Jerzy Radecki
Polish Academy of Science, Poland
Here we report on electrochemical genosensors devoted for detection of influenza virus H5N1 gene sequence. Using ssDNA
decorated with redox active units such as Co(II)-porphyrin or 3-iron bis (dicarbiollide), the detection limit in the fM range has
been achieved. The strategies based on dipyrromenthene Cu(II) redox active monolayer or phenanthroline-Epoxy-Fe(III) complexes
have been also applied for the development genosensors destined for detection of DNA as well for RNA derived from Avian Influenza
viruses. They have been working based on the new “ion barrier switch-off ” mechanism of analytical signal generation. To face
of the need of systems for simultaneous determination of few markers of one disease coming from medical diagnosis, we have
developed a novel dual DNA electrochemical sensor with “signal-off ” and “signal-on” architecture for simultaneous detection of two
different sequences of DNA derived from Avian Influenza Virus type H5N1 by means of one electrode. Two sequences of ssDNA
characteristic for hemagglutinin decorated with ferrocene and characteristic for neuraminidase decorated with methylene blue were
immobilized covalently together on the surface of one gold electrode. Taking into account the excellent analytical parameters of
genosensors presented such as good sensitivity, selectivity and very low sample consumption, they could be recommended for future
wide application for medical diagnostic as well as environmental control.
Biography
Jerzy Radecki is the Professor of Analytical Chemistry and currently working as Head of Department of Biosensors of IARFR PAS in Olsztyn. His research interest concerns
the developing of new sensors and biosensors based on the intermolecular recognition processes occurring at the border of the aqueous and organic phase. Particularly,
he is interested in functionalization of surface of solid electrodes with “host” molecules, which are responsible for “guest” molecules (analytes) recognitions. He is working
on not only analytical aspects of developed sensors but on the elaboration of the mechanism of analytical signal generation as well.
Jerzy Radecki, J Infect Dis Ther 2016, 4:6(Suppl)