influenza-2016_scientifictracks-abstracts

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Volume 4, Issue 6(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 29
Notes:
Influenza 2016
September 12-13, 2016
conferenceseries.com
Influenza
September 12-13, 2016 Berlin, Germany
2nd International Conference on
Electrochemical immunosensors: Universal tools for rapid detection of viruses
Hanna Radecka
Polish Academy of Science, Poland
Here, we report examples of successful developing of several type of immunosensors destined for the detectionofHighly-Pathogenic
Avian Influenza type H5N1 virus (HPAI) spreading among wild and domestic birds. The immunosensor were developed by the
successive modification of gold as well as glassy carbon electrodes. The whole antibody or their fragments have been applied as the
sensing elements. The complex between virions and specific antibody adsorbing on a surface of an electrode forms an insulating
layer. This phenomenon, which is a base of ion-channel mimetic type of immunosensors, can be monitored by the electrochemical
impedance spectroscopy (EIS) in the presence of [Fe(CN)6]3-/4- as a redox marker. The another type of immunosensors are based on
redox active layers incorporated di-pyrromethene-Cu(II). The changes of electrochemical parameters of redox centers upon target
analyte binging are the base of analytical signal generation. The both type of immunosensors displayed better sensitivity towards
viruses as well as antibodies in comparison to ELISA; they are also very selective. The matrix from hen sera has no influence on the
immunosensors performance. In addition, very small analyzed sample volumes (10 µl) are needed. After miniaturization, they keep
excellent analytical parameters. Therefore, immunosensors presented could be recommended for the direct electrochemical detection
of viruses as well as antibodies in the natural physiological samples.
Biography
Hanna Radecka was graduated from the Department of Chemistry of Nicolaus Copernicus University in Torun in 1978. She was a Visiting Scientist at the Hokkaido
University in Sapporo and at the University of Tokyo. Since 1998, she is working at Department of Biosensors of the Polish Academy of Sciences in Olsztyn. In 2011 she
has received the title of Professor of Analytical Chemistry and was nominated as the Head of Laboratory of Bioelectroanalysis. Currently she is working on the development
of the new biosensors for determination of avian influenza viruses, possible biomarkers of Alzheimer’s and other neurodegenerative diseases present in human plasma.
Hanna Radecka, J Infect Dis Ther 2016, 4:6(Suppl)