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Volume 6
Journal of Oral Hygiene & Health
Oral Health Meet 2018
November 29-30, 2018
Page 43
Notes:
conference
series
.com
November 29-30, 2018 Bali, Indonesia
International Conference on
Oral Health and Dental Medicine
Changes of salivary MMP-3 and TIMP-1 levels in periodontal therapy with
Pelargonium sidoides
DC. active
substances
Rasa Baniene
Lithuanian University of Health Sciences, Lithuania
Statement of the Problem:
Periodontitis is a chronic inflammatory
disease affecting the supporting structures of the teeth. This disease affects
more than 10% of the population and is the most common cause of tooth
loss worldwide. Periodontitis is caused by bacteria that adhere to and grow
on the tooth's surfaces, along with an over-increased immune response
against these microorganisms and induced the activation of the molecular
cascade of inflammation. Biologically active compounds of
Pelargonium
sidoides
root extract (PSRE) demonstrated possibility to modulate bacterial virulence, stimulate host immune responses and
reduced inflammation. Thus, they are coming under considerable scrutiny as potentially new non-aggressive therapeutic agents
for managing periodontal infections. The purpose of this study is to evaluate changes in molecular markers MMP-3, TIMP-1
in association with clinical signs of periodontitis and assess the protective effect of
Pelargonium sidoides
root extract (PSRE).
Methods:
Saliva from 20 heathy persons (control group), 20 periodontitis patients and 20 periodontitis patients, treated with
PSRE were collected using Salivette
saliva sample collection kit. Samples were centrifuged at 3650 rpm, aliquoted and stored
at -80
o
C until analysis. Amount of MMP-3 and TIMP-1 in saliva of periodontitis patients and healthy persons was evaluated
using commercial ELISA kits.
Results:
MMP-3 concentration was significantly higher in saliva of periodontitis patient's group as compared to healthy
persons. After treatment with PSRE, MMP-3 level in patient group saliva decreased and returned to control group level. TIMP-
1 in periodontitis patient's saliva was significantly lower as compared to healthy persons. After treatment with PSRE, TIMP-1
concentration in patients group saliva increased significantly as compared with TIMP-1 concentration in the same group
before treatment.
Conclusion:
Biologically active compounds of
Pelargonium sidoides
root extract decreased inflammation and protected
periodontal tissue.
Biography
Rasa Baniene is a professor of Biochemistry at the Lithuania Lithuanian University of Health Sciences, Department of Biochemistry. Rasa Baniene is specializing
in investigation of potential biomarkers in saliva for oral cancer detection and in analysis of molecular mechanisms of the regulation of energy transformation in the
human tissue. She published over 50 peer reviewed journal publications, 4 book chapters and she has managed international and national projects in the above
mentioned areas.
rasa.baniene@lsmuni.ltRasa Baniene, J Oral Hyg Health 2018, Volume 6
DOI: 10.4172/2332-0702-C2-011