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Pain Medicine 2017
October 19-20, 2017
Volume 6, Issue 6 (Suppl)
J Pain Relief, an open access journal
ISSN: 2167-0846
October 19-20, 2017 San Francisco, USA
4
th
International Conference on
Pain Medicine
Pathophysiology of migraine: What does calcitonin gene-related peptide do in early stage of migraine?
Minyan Wang
Xi’an Jiaotong-Liverpool University, China
M
igraine is a severe, episodic, unilateral headache lasting hours to days and affects an estimated 16% of the population worldwide.
Cortical Spreading Depression (CSD) is known to be the substrate of migraine with aura and may also lead to migraine like
behavior by potentiation of inflammatory responses. Calcitonin-gene related peptide (CGRP) receptors play a crucial role inmediating
the magnitude of CSD in rat cortical slice. However, how central CGRP is involved in early stage of migraine remains far from clear.
This study aimed to examine if central CGRP could be induced by CSD and if CGRP and its receptors contribute to CSD genesis
and propagation. CSD was induced and monitored both in vitro using intrinsic optical signal and in vivo using electrophysiology
approach. Quantitative PCR and ELISA were used to quantify CGRP mRNA and peptide levels. The data demonstrated multiple
but not single CSD, significantly increase CGRP mRNA and peptide in discrete regions of ipsilateral cortices and subcortical region.
In addition, inhibition of both CGRP and CGRP receptors markedly prolonged CSD latency and reduced CSD magnitude in chick
retina and mouse cortical slice, which actions on CSD were blocked by exogenous CGRP. Furthermore, reduction of CSD by CGRP
receptor inhibition was also observed in vivo. These findings uncover a previously unknown role of cortical CGRP in mediating CSD,
suggesting CGRP contributes to migraine pathophysiology and other CSD associated diseases.
minyan.wang@xjtlu.edu.cnJ Pain Relief 2017, 6:6 (Suppl)
DOI: 10.4172/2167-0846-C1-018