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conferenceseries
.com
Volume 7
Pharmaceutical Regulatory Affairs: Open Access
ISSN: 2167-7689
Pharma Europe 2018
May 07-09, 2018
May 07-09, 2018 | Frankfurt, Germany
15
th
Annual European Pharma Congress
TrichiliasinenoidsA-C, three novel 6, 7-secomexicanolide limonoids with a 7, 29-linkage from
Trichilia
sinensis
Donghua Cao
Chinese Academy of Sciences, China
M
eliaceous limonoids, characteristic chemical markers of the
Meliaceae
family are natural products with both fascinating
structures and potential bioactivities that have attracted interest from natural products chemists and synthetic chemists
in the past half century. As part of a continuing search for structurally interesting and biologically important limonoids from
the
Meliaceae
family, the leaves and twigs of
Trichilia sinensis
collected from Xishuangbanna, Yunnan province of China were
investigated.
Trichilia sinensis
Bentv, a shrub, is native to the south of China and Vietnam, having traditional applications in the
treatment of several diseases such as abdominal pain caused by
Ascaris lumbricoides
, chronic osteomyelitis, scabies, and eczema
in folk medicine. The three novel rearranged mexicanolide-type limonoids (trichiliasinenoids A-C) with an unprecedented
C-29-C-7 connecting carbon skeleton formed by migration of C-7 fromC-6 to C-29 of a mexicanolide-type limonoid precursor
were isolated from the leaves and twigs of
Trichilia sinensis
. Their structures were assigned by spectroscopic analysis, and the
absolute configurations were determined by X-ray crystallography and CD calculation. A possible biosynthetic pathway of
trichiliasinenoids A was also proposed. The three new limonoids were evaluated for their cytotoxic activity against human
myeloid leukaemia (HL-60), hepatocellular carcinoma (SMMC-7721), lung cancer (A-549), breast cancer (MCF-7), and colon
cancer (SW480) cell lines by MTS assay. Trichiliasinenoid B showed cytotoxicity against HL-60 cells, SMMC-7721 with an
IC
50
value of 5.2
µ
M and 30.6
µ
M, respectively, whereas other limonoids were inactive and comparable to the cisplatin positive
control (IC50: 1.1–17.3
µ
M).
caodonghua@xtbg.ac.cnPharmaceut Reg Affairs 2018, Volume 7
DOI: 10.4172/2167-7689-C1-031