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Volume 7, Issue 4 (Suppl)
Clin Exp Pharmacol
ISSN: 2161-1459 CPECR, an open access journal
Pharmacology Congress 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
8
th
World Congress on
Pharmacology and Toxicology
Genistein inhibition of OGD-induced neuronal death
in vitro
experiment
Yan-qiang Liu
Nankai University, China
I
n the present study, we established an
in vitro
model of hypoxic-ischemia via exposing PC12 cells and primary neurons of
newborn rats to oxygen-glucose deprivation (OGD) and observing the effects of genistein, a soybean isoflavone on hypoxic-
ischemic neuronal viability, apoptosis, voltage-activated potassium (K
v
) and sodium (Na
v
) currents and glutamate receptor
subunits expression. The results indicated that OGD exposure reduced the cell viability, increased apoptosis, decreased the
GluR2 expression and decreased the voltage-activated potassium currents in PC12 cells and genistein partially reversed the
effects induced by OGD. In primary neurons, OGD exposure reduced the viability and increased the apoptosis of brain neurons.
Meanwhile, OGD exposure caused changes in the current-voltage curves and current amplitude values of voltage-activated
potassium and sodium currents. OGD exposure also decreased GluR2 expression and increased NR2 expression. However,
genistein at least partially reversed the effects caused by OGD in primary neurons. The results suggest that hypoxic-ischemia
caused neuronal apoptosis/death is related to an increase in K
+
efflux, a decrease in Na
+
influx, a down-regulation of GluR2 and
an up-regulation of NR2. Genistein may exert some neuroprotective effects via the modulation of K
v
and Na
v
currents and the
glutamate signal pathway mediated by GluR2 and NR2.
Biography
Yan-qiang Liu has completed his PhD in Nanjing Agricultural University and Postdoctoral study in Chinese Military Medicine Academy, also visiting and co-
operative studies in Pisa University. He is also the Professor of College of Life Sciences Nankai University, China. He has published more than 80 papers in
reputed journals and has been serving as an Editorial Board Member of Acta Nutrimenta Sinica and the referee of many academic journals.
liuyanq@nankai.edu.cnYan-qiang Liu, Clin Exp Pharmacol 2017, 7:4 (Suppl)
DOI: 10.4172/2161-1459-C1-020