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.com
Volume 8
Journal of Clinical Trials
Pharmacy and Pharmacovigilance 2018
July 16-17, 2018
July 16-17, 2018 Sydney, Australia
Joint Event on
Global Pharmacovigilance and
Advanced Pharmacy
Electro-membrane extraction combined with capillary electrophoresis for the determination of
Metoclopramide and Ondansetron in urine samples
Ehsan Sadeghi and Ali Reza Fakhari
Shahid Beheshti University, Iran
E
lectro-Membrane Extraction (EME) is a sample preparation technique in pharmaceutical, chemical, clinical and environmental
analysis. This technique uses electro-migration across artificial liquid membranes for selective extraction of analytes and sample
enrichment from complex matrices. This method has many advantages such as simplicity, rapid, low-cost, low LOD, high pre-
concentration factor and high recovery. In the present work, simultaneous pre-concentration and determination of two basic drugs
namely Metoclopramide (MCP) and Ondansetron (OSN) were studied using EME as a suitable extraction method, followed with
Capillary Electrophoresis (CE) using Ultraviolet (UV) detection as separation technique. The drugs were extracted from 4 ml sample
solutions, through a Supported Liquid Membrane (SLM) consisting 2-Nitrophenyloctylether (NPOE) impregnated in the walls of a
polypropylene hollow fiber and into a 20μL acidic aqueous acceptor solution resent inside the lumen of the hollow fiber with a potential
difference applied over the SLM. The variables of interest, such as chemical composition of the organic liquid membrane, stirring speed,
extraction time and voltage, pH of donor and acceptor phases and salt effect in the EME process were investigated and optimized.
Under optimal conditions NPOE as SLM, stirring rate of 1000 rpm, 200V potential differences, 20min as the extraction time, acceptor
phase HCl (pH 1.0) and donor phase HCl (pH 1.5). After the micro-extraction process, the extracts were analyzed by CE with optimum
conditions phosphate running buffer (pH 2.0), applied voltage of 20kV and 25 °C. Under the optimum conditions, Limits of Detection
(LOD) and quantification (LOQ) for MCP and OSN were 2.31-2.68 and 7.72-8.91 ng mL
-1
, respectively. Pre-concentration factor and
RSD for five replicates of each drug were calculated to be 200 and 4.06-3.93, respectively. Finally, the applicability of this method was
studied by the extraction and determination of these drugs in urine samples with recovery percentages of 87-92%.
sadeghiehsan88@gmail.comJ Clin Trials 2018, Volume 8
DOI: 10.4172/2167-0870-C2-026