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Volume 6
Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029
Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6
th
Global Summit on Plant Science
Potentiometry in antioxidant activity evaluation of microsuspensions, extracts and living plants
Brainina Kh Z, Stozhko N Yu, Buharinova M A
and
Hamzina E I
Ural State University of Economics, Russia
Antioxidants are a large group of scavengers of reactive oxygen and nitrogen active species in living organism, that are an
integral part of the body's antioxidant defense system, which prevents (or reduces) the development of oxidative stress. Plants
are themain source of antioxidants.The potentiometricmethod of Antioxidant Activity (AOA) estimation is themost attractive.
The method is based on the interaction of antioxidants with components of the mediator system K
3
[Fe(CN
6
)]/K
4
[Fe(CN
6
)].
Shift of potential of mediator system when analysed sample introduced into it serves as source of information. The purpose of
this study is to develop a potentiometric method for estimating plant AOA. The objects of the study were dried leaves of fruit
and berry plants growing in the Urals (
Fragária, Ríbes nígrum, Ríbes úva-críspa
) and living leaves of indoor plants (
Crassula,
Schlumbergera, Sansevieria
). AOA of plant extracts and microsuspensions were evaluated without separating the liquid and
solid phases, as well as the living leaves without additional sample preparation. It was shown that the water extract obtained
for 20 minutes at 80°C has the largest AOA. The use of a water-alcohol extractant (1:1 by volume) increases AOA of plant
extracts and microsuspensions by a factor of 1.5–2, which is associated with the involvement of both water- and fat-soluble
antioxidants in the zone of the signal-forming reaction. For the first time, the AOA study of a living leaf was performed and it
was shown that the AOA of the pulp on the longitudinal section of the leaf plate is approximately an order of magnitude higher
than the AOA of the skin of the leaf. The duration of AOA assessment of a living leaf without preliminary sample preparation
does not exceed 20 minutes, which is 3 times faster than analysis of plant extracts and microsuspensions, including additional
operations of grinding, sifting and heating of plant material. Simplicity and speed, the lack of a multi-stage and long-term
preparation of the sample when assessing the AOA living leaf make this method very promising for both laboratory and non-
laboratory analysis.
Figure 1:
Effect of extraction temperature on AOA
Brainina Kh Z et al., J Plant Biochem Physiol 2018, Volume 6
DOI: 10.4172/2329-9029-C1-003