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.com
Volume 6
Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029
Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6
th
Global Summit on Plant Science
White birches buds: Chemical composition and perspectives for therapy of tumors
Valery A Isidorov
1
, Łukasz Szoka
2
, Marcin Stocki
1
and
Jolanta Nazaruk
2
1
Białystok University of Technology, Poland
2
Medical University of Białystok, Poland
B
irch buds (
Betulae gemmae
) are widely used inRussian andChinese traditional medicine not only as a diuretic and diaphoretic
agent but also as an antiseptic, anti-inflammatory and analgesic. In spite of long history of therapeutic use of birch buds in
folk medicine the existing information about their chemical composition and pharmacological effects is insufficient. The present
study was designed to investigate the chemical composition of buds from two species of white birch and the
in vitro
cytotoxic
effect of extracts from these sources on selected tumor cells. Extracts of
Betula pubescens
Ehrh. and
Betula pendula
Roth buds
were obtained using three different methods like supercritical carbon dioxide extraction by washing of exudate covering the
whole buds and extraction of milled buds with diethyl ether. The chemical composition of extracts was investigated by GC-
MS method; cytotoxicity was determined by MTT assay using cancerous Ishikawa, MCF-7, MDA-MB-231, DLD-1, HeLa and
normal skin fibroblasts as target cells. Anticancer drugs cisplatin, melphalan and 5-fluorouracil were used as reference agents. As
the result of GC-MS investigation, 150 substances of different classes were identified in all extracts. The chemical composition
of
B. pubescens
and
B. pendula
buds was highly contrasting. Bud extracts of the former have contained relatively high quantity
of sesquiterpenoids and flavonoids, while the latter main components of extracts were from the triterpenoids. The results of
biological assay indicated that birch bud extracts has demonstrated the concentration dependent and differential cytotoxicity
which is rather weak at 30 μg/mL, moderate to high at 100 μg/mL and very high at 300 μg/mL. The highest cytotoxic activity had
bud exudates obtained from both betulae species and SFE extract from
B. pendula
exert greater cytotoxic effects than SFE extract
from
B. pubescens
and ether extracts. The rich composition of birch buds suggests possible wider spectrum of their biological
activity than showed old commendations. It was stated that birch bud extracts could be a promising source of compounds with
cytotoxic activity against various cancers.
v.isidorov@pb.edu.plJ Plant Biochem Physiol 2018, Volume 6
DOI: 10.4172/2329-9029-C1-003