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Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal
Page 55
Notes:
Separation Techniques 2016
September 26-28, 2016
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
Arsenic speciation in edible marine algae: Presence of water and lipid-soluble arsenic compounds
Sara Garcia Salgado1, Georg Raber2, Kevin A Francesconi2 and M Angeles Quijano1
1Universidad Politecnica de Madrid, Spain
2Karl-Franzens University Graz, Austria
Nowadays, there is considerable interest in arsenic speciation in food products due to the different toxicity exhibited by the
different arsenic compounds. This is accentuated in the case of marine algae, because they contribute substantial amounts
of arsenic to the human diet and their consumption is increasing due to their properties as food additives, nutritional values
and suggested medical applications. Organic arsenic compounds are abundant in marine ecosystems. Although most of the
arsenic compounds identified so far have been water-soluble species, the early work on arsenic marine chemistry focused on
lipid-soluble compounds. In 1988, an arsenolipid was first rigorously characterized and identified as an arsenosugar-containing
phospholipid in algae. Subsequently, several arsenic-containing fatty acids and hydrocarbons have been discovered in different
fish products, which origin was presumed to be algae. In this work, we report the water and lipid-soluble arsenic compounds
found in 9 commercially available edible marine algae from Japan and Spain. The extraction of water-soluble arsenic species
was performed by microwave-assisted extraction, using deionized water as extracting agent, and they were determined by
HPLC-(UV)-HG-AFS. Lipid-soluble arsenicals were extracted by mechanical shaking with a (2:1; v/v) chloroform/methanol
mixture, purified by SPE on home-made silica columns and determined by online HPLC-ICPMS/ESMS analysis. 6 water-
soluble arsenic species, comprising DMA, As(V) and 4 arsenosugars (glycerol, phosphate, sulfonate and sulfate sugars), as well
as 14 lipid-soluble arsenic species (3 arsenic-containing hydrocarbons and 11 arsenosugar-containing phospholipids), were
found in the water and chloroform extracts, respectively, of the edible marine algae analyzed.
Biography
Sara Garcia Salgado has completed her PhD in 2013 from Technical University of Madrid, Spain. She is an Assistant Professor in this University and Deputy
Director for External Relations at School of Civil Engineering. She has published 10 papers in reputed journals and published two books.
Sara Garcia Salgado et al., J Chromatogr Sep Tech 2016, 7:6(Suppl)