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Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal
Page 66
Notes:
Separation Techniques 2016
September 26-28, 2016
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
Anion exchange chromatography in lignocellulosics analysis
Nico Anders1, Harald Humann1, Bernd Langhans1 and Antje Spieß1, 2
1RWTH Aachen University, Germany
2TU Braunschweig, Germany
C omplex liquid samples such as lignocellulose hydrolyzates which contain both polar and nonpolar analytes as well as
monomeric and oligomeric compounds are hard to analyze and require usually more than one analytical separation
mechanism. In contrast, the reversed-phase mechanism anion exchange chromatography (AEC) allows for a separation of
divergent analytes based on the acidity of the resulting anion. Thus, the aim of this study was to investigate the potential of
AEC to analyze lignocellulosic biomass hydrolyzates completely using one analytical separation mechanism, as lignocellulose
hydrolyzates contain a lot of potential anions with a variation in polarity and size. Therefore, the potential of generating anions
from aldehydes, alcohols and phenols was investigated using an alkaline eluent. Additionally, the concentrations of aldehydes,
alcohols and phenols derived from lignins were measured simultaneously with monosaccharides, oligosaccharides and uronic
acids derived from cellulose, hemicellulose and pectin. Thus, parameters such as column temperature, eluent composition
and chromatographic run time were examined. The final chromatographic method was set to a column temperature of 40°C,
an eluent flow of 1 ml/min and an eluent consisting of sodium acetate and sodium hydroxide as well as ultrapure water. This
method allows for a complete characterization of lignocellulose hydrolyzates with limit of detections in the range of 0.014
mg/L for 2,6-dimethoxyphenol and 21.9 mg/L for 4-methoxybenzyl alcohol. Finally, this method was used to characterize 17
hydrolyzates from lignocellulosic biomass simultaneously for cellulose, hemicellulose, lignin and pectin derived degradation
products.
Biography
Nico Anders has been working in the field of analysis and renewables since 2009. He has obtained his PhD from the TU Braunschweig in the group of Prof. Dr.
Vorlop in Technical Chemistry. Since 2013, he is working as a Junior Research Group Leader in the Aachener Verfahrenstechnik at the RWTH Aachen University.
His research interests are analysis of lignocellulosic biomass, green analytical chemistry, conversion of lignocellulosic biomass and chromatographic separation.
Nico Anders et al., J Chromatogr Sep Tech 2016, 7:6(Suppl)