separation-techniques-2016_scientifictracks-abstracts

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Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal
Page 69
Notes:
Separation Techniques 2016
September 26-28, 2016
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
Robustness test of the chromatographic method for the quantification of chlorogenic acid in
coffee brew
Jong Sup Jeon
Gyeonggi Province Institute of Health and Environment, Republic of Korea
C offee has been for decades the most commercialized and widely consumed beverage in the world. Coffee beans contain a
large variety of substances, which in many cases are biologically active such as caffeine and chlorogenic acids. Chlorogenic
acids are water-soluble phenolic components of coffee and other plants formed by the esterification of certain trans-cinnamic
acids, such as caffeic, ferulic and p-coumaric acids with quinic acid. The main subgroups of chlorogenic acid isomers in coffee
are the caffeoylquinic acids, feruloylquinic acids, dicaffeoylquinic acids and in smaller amounts p-coumaroylquinic acids. In
this study, we carried out robustness evaluation for method validation of quantification of chlorogenic acid in coffee brew using
Youden’s test. It was possible to determine the effect of each analytical parameter in the final analysis results. Seven analytical
factors were selected and small variations were induced in the nominal values of the method. Then, 8 runs were performed
aiming to determine the influence of each parameter in the final result. The 7 factors are: Concentration of KH2PO4 in mobile
phase, column temperarture, flow rate, wavelength of detector, column supplier and initial mobile phase composition. Standard
deviation of the differences Di (SDi) was calculated and that value was not larger than the standard deviation of the method
carried out under within-laboratory reproducibility conditions. The experimental t values resulted below the 2-tailed t-critical
value for all 7 factors. The tested procedure proved to be fairly robust since minor fluctuations in the operative parameter that
can occur during the routine application of the method do not significantly affect its performance characteristics.
Biography
Jong Sup Jeon has completed his PhD from College of Veterinary Medicine, Konkuk University, Republic of Korea. He is a DVM and Research Scientist for the
Public Health in Gyeonggi Province, Institute of Health and Environment, Republic of Korea. He has published variable domestic and international papers (subjects:
Antibiotics, hazardous materials in food and functional ingredients in cosmetics) in reputed journals and served as a co-worker in various field.
Jong Sup Jeon, J Chromatogr Sep Tech 2016, 7:6(Suppl)