separation-techniques-2016_scientifictracks-abstracts

Volume 7, Issue 6(Suppl)

J Chromatogr Sep Tech

ISSN: 2157-7064 JCGST, an open access journal

Page 70

Notes:

Separation Techniques 2016

September 26-28, 2016

conferenceseries

.com

Separation Techniques

September 26-28, 2016 Valencia, Spain

International Conference and Expo on

Purification and characterization of lipase enzyme from

Lactobacillus brevis

and immobilization

onto magnetic florisil NPs

Hayrunnisa Nadaroglu

, Seyedh Sara Soleimani

and

Zulal Kesmen

Ataturk University, Turkey

Erciyes University, Turkey

ipases (E.C.3.1.1.3; triglycerol acylhydrolases) are enzymes catalyzing reversible hydrolysis of animal and vegetable

oils under normal conditions. Besides, they also catalyze reactions such as esterification and transesterification. In this

study, a new lipase enzyme was isolated from

Lactobacillus brevis

and immobilized onto modified florisil with iron NPs and

the usability of free and immobilized lipases as a detergent additive material was investigated. Lipase enzyme was purified

using ammonium sulphate precipitation, DEAE-Sephadex ion-exchange chromatography and sephacryl S200 gel filtration

chromatography techniques. Its molecular mass was determined to be 57 kDa by SDS-PAGE and gel filtration chromatography.

Purified lipase was immobilized onto magnetic florisil NPs and determined immobilization conditions. Also immobilized

lipase characterization was done using SEM, FTIR and XRD techniques. Immobilized lipase showed good thermo-stability and

retained its activity at 80%, than free lipase enzyme at 60°C. The free and immobilized lipase enzymes were most stable in the

alkaline pH. Also, immobilized lipase had more stability towards metal ions than free lipase enzyme. Washing performances

of some detergents formulation were done and maximum percentage of olive oil was removed by the immobilized lipase

than commercial detergents. The study on oil stain removal from cotton cloth indicated that oil removal was superior in the

presence of immobilized lipase and immobilized lipase with detergent than the detergent alone.

Biography

Hayrunnisa Nadaroglu has completed her PhD and MSc from Ataturk University, Graduate Institute of Sciences, Department of Biochemistry (Erzurum, Turkey)

in Bioorganic Reactions using purified carbonic anhydrase isoenzymes. She is a Scientific Expert in process development on bioremediation of waste water,

some nano-biotechnological applications onto environmental pollution and some industrial enzyme applications onto food technology (clarification of fruit juice,

hydrolyzation of phytate etc.). She has published more than 120 papers in the field of biochemistry, food technology and nano-biotechnology in journals and

conferences.

hnisa25@atauni.edu.tr

Hayrunnisa Nadaroglu et al., J Chromatogr Sep Tech 2016, 7:6(Suppl)

http://dx.doi.org/10.4172/2157-7064.C1.019