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conferenceseries
.com
Volume 8, Issue 9 (Suppl)
J AIDS Clin Res
ISSN: 2155-6113 JAR, an open access journal
STD Asia Pacific 2017
October 23-25, 2017
OCTOBER 23-25, 2017 OSAKA, JAPAN
7
TH
ASIA PACIFIC
STD and Infectious Diseases Congress
Distribution of AdeABC efflux system genes in
Acinetobacter baumannii
isolates isolated from blood
cultures of hospitalized patients and their relationship with Carbapenem resistance
Hamza Ari
1
, Okanaydogan
1
, Mehmet Demirci
2
and Fatma Koksal Cakirlar
1
1
Cerrahpasa Faculty of Medicine, Turkey
2
Beykent University, Turkey
Statement of the Problem:
Acinetobacter baumannii
is an important nosocomial pathogen leading various infections. The
major efflux mechanism associated with carbapenem resistance in
A. baumannii
is the chromosomally encoded tripartite
efflux system, AdeABC. Its’ over expression is regulated by adeRS genes which encode two-component regulatory system. This
study investigated the distribution of the AdeABC efflux pump genes and their relationship to carbapenemases production in
A. baumannii
isolates isolated from blood cultures of hospitalized patients.
Methodology & Theoretical Orientation:
A total of 97
A. baumannii
were isolated from blood cultures of hospitalized
patients in Cerrahpasa medical faculty hospital in Istanbul, Turkey. The Phoenix Automated System was used to identify
isolates and determine antibiotic susceptibility. AdeRS mutations and adeB gene expression of drug efflux genes were analyzed
by sequencing and qPCR, respectively.
Findings:
Of our 97 isolates, 61 were carbapenem resistant. Resistance rates of carbapenem resistant
A. baumannii
(CRAB)
isolates were found to be 100% for ceftazidime, 96.7% for cefepime, piperacillin-tazobactam, ciprofloxacin, trimethoprim-
sulfamethoxazole, 86.8% for amikacin, 75.4% for gentamicin and netilmicin. All isolates were positive for the adeB genes.
Significant over expression (3.45-52.18) of adeB was observed in 49 CRAB isolates whereas only 12 CRAB isolates (0.23-0.54)
and non-CRAB isolates (0.109-0.783) had less increased levels. In 80.3% of CRAB isolates were positive for the adeRS genes.
The p.Val120Ile change in the AdeR amino acid sequence was determined in 42.8% in adeB-overexpressing CRAB isolates.
The p.His158Leu and p.Pro116Ser changes were found in 36.7% of these isolates. None of the CRAB isolates had p.Val120Ile,
p.His158Leu and p.Pro116Ser changes. In the AdeS amino acid sequence, p.Gly293Ser, p.Leu105Phe and His227Asp changes
were most commonly found in adeB-overexpressing CRAB isolates, whereas pGly293Ser change was detected in only %8 of
non-CRAB isolates.
Conclusion:
The results showed that were significantly associatedwith between theAdeABC efflux systemand both carbapenem
and multiple drug resistance in our
A. baumannii
isolates.
Biography
Fatma Koksal Cakirlar has been working as lecturer in Istanbul University Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey and
she works on hospital infections, drug resistance, nano-technology and rapid diagnostic devices.
fatma.koksal@istanbul.edu.trFatma Koksal Cakirlar et al., J AIDS Clin Res 2017, 8:9 (Suppl)
DOI: 10.4172/2155-6113-C1-021