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.com
Volume 8, Issue 9 (Suppl)
J AIDS Clin Res
ISSN: 2155-6113 JAR, an open access journal
STD Asia Pacific 2017
October 23-25, 2017
OCTOBER 23-25, 2017 OSAKA, JAPAN
7
TH
ASIA PACIFIC
STD and Infectious Diseases Congress
Development of biosensor platforms for rapid detection of
Escherichia coli
from clinical isolates
Hatice Nur Halipci Topsakal
1
, Ashabil Aygan
2
, Ahmet Altindal
3
, Cigdem Oruc
3
and Fatma Koksal Cakirlar
4
1
Istanbul Ayvansaray University, Turkey
2
Kahramanmaras Sutcu Imam University, Turkey
3
Yildiz Technical University, Turkey
4
Cerrahpaşa Faculty of Medicine, Turkey
Background & Aim:
Today, we face with increasing resistance of pathogenic bacteria that cause infection in the hospital
and community. Therefore, there is a growing need for selective recognition of bacteria in clinical samples. Biosensors-based
analysis systems used to the diagnosis of bacteria have been one of the most important techniques due to demonstrated low cost,
fast response, high sensitivity and high selectivity. DNA aptamers have a promising role and they have inherent advantages in
stability and facility of generation and synthesis. In this study, it is aimed to develop ssDNA containing biosensor array which
can give rapid results for identification of intended bacteria
Escherichia coli
.
Methodology & Theoretical Orientation:
Cell-based Systematic Evolution Of Ligands By Exponential Enrichment (SELEX)
to isolate an
E. coli
-aptamer that shows strong binding was performed. After selection process one of the ssDNA aptamer was
chosen that strongly binds to
E. coli
. The selected aptamer was placed on two different biosensor platforms containing silica
and Quartz Crystal Microbalance (QCM) materials. Binding experiments of
E. coli
to biosensor platforms were carried out
using impedance spectrometry and frequency analyzer.
Findings:
The fluorescently labeled aptamer was purchased commercially and it was detected that the aptamer was ligated with
E. coli
using a fluorescent microscope. Whether or not our aptamer ligates to 50
E. coli
strains isolated from blood cultures of
hospitalized bacteraemic patients were examined, for specificity and susceptibility. We showed that our fluorescently labeled
aptamer binds to a variety of
E. coli
strains at a certain level.
Conclusion & Significance:
With biosensor based medical diagnostic system, identification could be done easier and faster
thus enable infection control, treatment and surveillance to be done more quickly and easily. In addition, early diagnosis and
treatment will reduce mortality, morbidity and high costs.
Biography
Hatice Nur H. Topsakal is a PhD candidate. She has been working as lecturer in Istanbul Ayvansaray University Plato Vocational School, Medical Laboratory
Department. She works on Microbiology, and interested in DNA technologies. She is a holder of scholarship from the Scientific And Technological Research Council
Of Turkey(Tubitak)
haticetopsakal@ayvansaray.edu.trHatice Nur Halipci Topsakal et al., J AIDS Clin Res 2017, 8:9 (Suppl)
DOI: 10.4172/2155-6113-C1-021