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.com
Volume 8, Issue 9 (Suppl)
J AIDS Clin Res
ISSN: 2155-6113 JAR, an open access journal
STD Asia Pacific 2017
October 23-25, 2017
OCTOBER 23-25, 2017 OSAKA, JAPAN
7
TH
ASIA PACIFIC
STD and Infectious Diseases Congress
Identification and Macrolide-Lincosamide-Streptogramin B (MLSB) resistance phenotypes and slime
production of coagulase-negative
Staphylococci
isolated from bloodstream infections in Turkey
Sinem Ozdemir, Okan Aydogan and Fatma Koksal Cakirlar
Cerrahpasa Faculty of Medicine, Turkey
Aim:
The aim of this study is identification and determination of the MLSB type resistance, antibiotic resistance patterns and
slime production of CoNS isolated from blood cultures of hospitalized patients with bacteremia.
Methodology & Theoretical Orientation:
Blood cultures were analyzed by the Bactec 9120 system. The identification and
antimicrobial resistance of the CoNS were determined by Phoenix automated system and MALDI-TOF MS. Antibiotic
susceptibilities were evaluated according to EUCAST. The slime production was evaluated with Congo red agar method.
Findings:
A total of 160 CoNS strains were isolated from blood samples of patients with true bacteremia who were hospitalized
in intensive care units and in other departments of Istanbul University Cerrahpasa Medical Hospital between 2015 and
2017. Among CoNS isolates,
Staphylococcus epidermidis
was the most prevalent species (51.2%) followed by
Staphylococcus
hominis
(20%),
Staphylococcus haemolyticus
(11.8%),
Staphylococcus capitis
(4.3%),
Staphylococcus saprophyticus
(1.8%),
Staphylococcus cohnii
(1.25%),
Staphylococcus lugdunensis
(0.6%),
Staphylococcus schleiferi
(0.6%),
Staphylococcus warneri
(0.6%) and
Staphylococcus pettenkoferi
(0.6%). Resistance to methicillin was detected in 80.5% of CoNS isolates. Methicillin-
resistant CoNS isolates (MRCoNS) were determined to be more resistant to antibiotics than methicillin-susceptible CoNS
isolates (MSCoNS). Resistance rates of MRCoNS and MSCoNS isolates to the antibacterial agents, respectively, were as follows:
Gentamicin 38.7% and 0%, erythromycin 83.5% and 32%, clindamycin 38.6% and 14.8%, trimethoprim-sulfamethoxazole
49.4% and 8.3%, ciprofloxacin 67.8% and 21.7% tetracycline 50% and 34.7%, rifampicin 42% and 0%, teicoplanin 4.9% and
0%. None of the isolates were resistant to vancomycin. The inducible MLSB, structural MLSB and efflux type resistance were
determined in 44.9%, 1.12% and 38.2% of MRCoNS and in 14.8%, 0% and 14.8% of MSCoNS, respectively. Slime production
was determined higher in MRCoNS.
Conclusion & Significance:
These results underline the importance of continuous surveillance and efforts to improve the
outcome of serious bloodstream.
Biography
Sinem Ozdemir, PhD, is working on hospital infections and drug resistance at Istanbul University Cerrahpasa Medical Faculty, Department of Medical Microbiology,
Istanbul, Turkey
sinemoz82@yahoo.com.trSinem Ozdemir et al., J AIDS Clin Res 2017, 8:9 (Suppl)
DOI: 10.4172/2155-6113-C1-021