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Volume 6, Issue 2 (Suppl) Virol-mycol
ISSN: 2161-0517 VMID, an open access journal Virology Asia 2017
May 11-12, 2017
May 11-12, 2017 Singapore
10th World Congress on
Virology and Mycology
Isolation, speciation and antifungal susceptibility testing of Candida isolates fromvarious clinical specimens
at a tertiary care hospital, Nepal
Sundar Khadka
National Public Health Laboratory, Nepal
Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threating invasive
diseases. Identification of candida up to species level and its antifungal susceptibility testing has paramount significance in the
management of candidal infections. CHROM agar media can be reliably used for speciation of Candida isolates which helps to
rapid identification of Candida species. The objective of the present study was to determine different species of Candida from
various clinical specimens and to determine antifungal susceptibility pattern of candida species to four antifungal agents namely
ketoconazole, fluconzole, miconazole, and clotrimazole. A total of 100 consecutive Candida isolates from various clinical samples
were studied. Growths on Sabouraud’s Dextrose Agar were evaluated for colony appearance, macroscopic examination, Gram
staining, germ tube test and urea hydrolysis test. They were further processed for Candida speciation on CHROM agar. Different
species of Candida were differentiated based on type of growth and color of isolates on CHROM agar media. Antifungal susceptibility
testing was performed and interpreted for all the isolates using disc diffusion method as recommended by Clinical and Laboratory
Standards Institute (CLSI) M44-A document. Out of 100 Candida isolates, Candida albicans (56%) was the most common species.
Among the non-albicans candida (NAC), Candida tropicalis (20%) was the commonest isolate followed by Candida glabrata (14%)
and Candia krusei (10%) respectively. Overall susceptibility pattern of Candida species to clotrimazole found to be more sensitive
(82%) followed by fluconazole (64%), miconazole (44%) respectively whereas ketoconazole was found to be more resistance (86%).
Candida albicans was the predominant species responsible for various candidal infections. Among commonly used antifungal drugs
clotrimazole, miconazole and fluconazole showed high sensitivity while ketoconazole was the least effective for both albicans and
non-albicans group. CHROM agar is a simple, rapid & inexpensive method for identification of Candida species and is suitable for
clinical laboratory with limited resources.
Biography
Sundar Khadka has completed MSc (Microbiology) from Institute of Medicine (IOM), Tribhuvan University Teaching Hospital, Nepal. He is currently working as
Microbiologist at HIV Reference Unit, National Public Health Laboratory, Nepal. He has published papers on HIV drug resistance, dermatophyte infection, etc.
Sundar Khadka, Virol-mycol 2017, 6:2 (Suppl)