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Volume 6, Issue 2 (Suppl) Virol-mycol
ISSN: 2161-0517 VMID, an open access journal Virology Asia 2017
May 11-12, 2017
Page 39
Notes:
conferenceseries.com
May 11-12, 2017 Singapore
10th World Congress on
Virology and Mycology
Issues in laboratory diagnosis of acute encephalitis syndrome
Statement of the Problem: Acute encephalitis syndrome is a group of neurologic manifestations caused by a plethora of
organisms including viruses, bacteria, fungi, parasites and toxins. There is a large amount of cross reactivity between the
different etiological agents. Also, besides Japanese Encephalitis Virus, no guidelines exist for establishing the diagnosis of
specific etiologic agents. This session deals with the methods available, practical problems, their advantages and disadvantages
for laboratory testing of agents of AES.
Methodology & Theoretical Orientation: An extensive literature search was done to summarize a comprehensive approach
for the laboratory investigations in the diagnosis of AES.
Findings: Laboratory diagnosis is generally established by testing the serum or CSF sample to detect virus specific IgM
antibodies. IgM antibodies are usually detectable 3 to 8 days after onset of illness and persist usually for 30 to 90 days. Therefore,
positive IgM antibodies may reflect a past infection or vaccination. Sample collected within 10 days of illness onset may not
have detectable antibodies and so Real Time PCR and antibody testing on a convalescent sample becomes important. For
patients with IgM antibodies, confirmatory neutralizing antibody testing should be performed. In fatal cases, nucleic acid
amplification, histopathology with immunohistochemistry, virus culture of autopsy tissues can also be useful.
Conclusion & Significance: The choice of test depends upon the prevalent etiological agent in a geographical region, patient’s
post-illness day of presentation to the health care centre, sample available and diagnostic facilities available. A laboratory
network should be established for better patient management and optimum utilization of resources.
Biography
Shantanu Prakash is PhD in field of virology and infectious diseases. He is basically involved with research, academics and in academics, research and patient manage-
ment. He has more than 25 publications, four patents and thousands of sequences submitted on NCBI of different viruses and bacteria and has been involved in many
intramural and extramural projects. He has experience in the field of designing molecular diagnostics assay for infectious diseases, molecular characterization & whole
genome sequencing of different viruses. Right now he is involved extensively on epidemiology & surveillance of AES in northern India with focus of newer emerging and
reemerging pathogens. The present study is based on several years of experience in serological and molecular techniques.
Shantanu Prakash
King George’s Medical University, India
Shantanu Prakash, Virol-mycol 2017, 6:2 (Suppl)