Open Access Scientific Reports

Your Research - Your Rights

Studies on Preparation of Buns Fortified with Germinated Horse Gram Flour

Research Article Open Access
Chandralekha Bhokre1, Pravin Udhavrao Ghatge*1, Girish Machewad2 and Ashok Rodge1
1Department of Food Chemistry and Nutrition, College of Food Technology, Marathwada Krishi Vidyapeeth, Parbhani, India
2Department of Food and Industrial Microbiology, College of Food Technology, Marathwada Krishi Vidyapeeth, Parbhani, India
*Corresponding authors: Pravin Udhavrao Ghatge
Department of Food Chemistry and Nutrition
College of Food Technology
Marathwada Krishi Vidyapeeth
Parbhani–431 402, India
Tel: +91-02452-222468
Fax: +91 02452-234150
E-mail: pughatge@indiatimes.com
 
Received June 07, 2012; Published August 10, 2012
 
Citation: Bhokre C, Ghatge PU, Machewad G, Rodge A (2012) Studies on Preparation of Buns Fortified with Germinated Horse Gram Flour. 2: 228. doi:10.4172/scientificreports.228
 
Copyright: © 2012 Bhokre C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Abstract
 
The horse gram is a cheapest source of protein, calcium and iron. Further the germination of horse gram seeds can reduce the anti-nutritional factors. The germination was carried out by washing, soaking (8 h), germination period (24 h), oven drying (500C) and ground into flour in hammer mill. The effect of germination on chemical characteristics of horse gram was found significant decrease (P>0.05) in protein, carbohydrate, fat and significant increase (P < 0.05) in moisture and ash content. Further value added product (buns) was prepared incorporation of (5%, 10%, 15% and 20%) with germinated horse gram flour (GHF) by straight dough method. The sensory evaluation of buns was carried out by a panel of ten trained using 9-point Hedonic scale. The sensory quality of bun were significantly decreased (P>0.05) for higher level of GHF and significantly increased (P < 0.05) for low level of GHF. The buns prepared by incorporation of GHF up to 10% were acceptable without affecting organoleptic quality.
 
Keywords
 
Buns; Germination; Chemical characteristics; Sensory quality
 
Introduction
 
The horse gram (Dolichos biflorus L.) commonly known as kulthi is a traditional unexploited tropical grain legume and well known for its hardiness, adaptability to poor soil and adverse climatic conditions. The horse gram is a cheapest source of protein. Further it is also rich in minerals such as calcium. The chemical composition is comparable with commonly cultivated legumes. Like other legumes, this is deficient in methionine and tryptophan. Horse gram is an excellent source of iron and molybdenum. Comparatively, horse gram seeds have higher trypsin inhibitor and hemagglutinin activities and polyphenols than moth bean seeds. Dehusking, germination, cooking, and roasting have been shown to produce beneficial effects on nutritional quality of the legume [1]. The germination is a simple method of food processing that result in increased nutritive value and decreases the phytates, tannin level and increases the availability of iron and calcium [2].
 
The buns are yeast leavened food like bread. Further the requirement of raw materials as well as the procedure in case of buns is similar to those of bread except buns are rounded and moulded with bun moulders. The wheat flour is major ingredient of bun and it is well reported that in fortifying wheat flour with high lysine material specially legume flour to increase the protein content [3]. Thus efforts have made to prepare germinated horse gram flour (GHF) to reduce the anti-nutritional factor and prepare buns with the incorporation of germinated horse gram flour to increase its protein content with acceptable organoleptic quality.
 
Materials and Methods
 
The horse gram seeds procured from the Project Coordinator, AICRP on Arid Legume, Jodhpur.
 
Preparation of GHF
 
The seeds were washed in tap water, soaked in the mercuric chloride solution (0.1%) for period (3- 4 min) and then washed thoroughly with distilled water until free from chloride ions. Further the seeds were soaked in distilled water of depth (3-4 cm) at room temperature for the period (8 h). The water was changed thrice and then seeds were placed in glass trays covered with wet muslin cloth and allowed to germinate for the period (24 h). The germination of the horse gram was carried out as per standard method [4]. The germinated horse gram seeds were dried in oven (500C) for period (5-6 h) .The dried germinated horse gram seeds was ground in hammer mill (Milcent, Anand. Gujarat. India).
 
Preparation of buns fortified with GHF
 
The prepared GHF was used for buns preparation. The buns were prepared by straight dough method (Figure 1). The recipe for preparation of buns fortified with GHF (Table 1). The buns were prepared by incorporation of GHF (Table 2).
 
Figure 1: Flow sheet for preparation of buns fortified with GHF.
 
Table 1: Recipe for preparation of buns fortified with GHF.
 
Table 2: The levels of GHF replaced with maida in the preparation of buns.
 
Determination of chemicals characteristics of horse gram and germinated horse gram seeds
 
The chemicals characteristics such as moisture content, carbohydrate, protein, lipid and ash were determined as per standard procedure [5].
 
Organoleptic evaluation
 
The sensory evaluation buns fortified with varying levels germinated horse gram flour on sensory quality of buns was carried out by trained panel of ten judges on a 9.0 point Hedonic scale [6].
 
Statistical analysis
 
The data generated in the experiments were recorded and subjected to statistical analysis using standard procedure [7]. The standard errors (SE) and critical differences (CD) at 5% level of significance were worked out for comparison of treatments and presented in the respective tables.
 
Results
 
The effect of germination on chemical characteristics of horse gram was found increased in moisture content and ash of horse gram from 10.18-10.40% and 2.88-3.10 respectively during germination the period (24 h) (Table 3). The protein content of horse gram was found decreased from 26.50-26.1%. This is due to utilization of amino acid and peptides during growth and increase in proteolytic activity [8]. The carbohydrate and lipid content were found slightly decreased during germination of the horse gram seeds. This is because of increase in amylase and lipase activity. The nonsignificant increase in ash content was observed.
 
Table 3: Effect of germination on chemical characteristics of horse gram seeds.
 
The sensory evaluation of buns fortified with GHF scored less than the control sample (Table 4). Further the sensory quality attributes scored less value for higher level of GHF and increased for low level of GHF fortified buns respectively. The buns fortified with GHF (5 10% and 15%) were acceptable than the buns fortified with GHF (20%). The buns fortified with GHF (20%) significantly affected (P < 0.05) the colour of crust and crumb, texture and flavour. Finally it can be concluded that the buns fortified with GHF were found to contain high amount of proteins, minerals and sensory qualities were found acceptable.
 
Table 4: Effect of germinated horse gram flour on sensory quality of buns.
 
 
References