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Volume 9
Chemical Sciences Journal
Asia Chemistry 2018
September 12-13, 2018
11
th
Annual Congress on
September 12-13, 2018 Singapore
Chemistry
Chem Sci J 2018, Volume 9
DOI: 10.4172/2150-3494-C5-030
Cytotoxicity and Phytochemical evaluation of
Pycnanthus angolensis
(Welw.) Warb dichloromethane
and Ethyl acetate stem bark extracts against HeLa cells
Ijeoma Solomon Okoro and Terrumun Amom Tor-Anyiin
University of Agriculture, Nigeria
C
ancer is one of the major life threatening diseases in the world today and its cell resistance to widely used chemotherapeutic
agents is gradually developed. Natural products, mainly isolated from medicinal plants, have been considered as valuable
sources for herbal anticancer drugs. The present study aimed to evaluate phytochemicals and
in vitro
cytotoxic activities of
the dichloromethane and ethyl acetate stem bark extracts from
Pycnanthus angolensis
(Welw.) Warb. on cancer cell line-
Human Cervix Adenocarcinoma (HeLa) cells. Dichloromethane and Ethyl acetate extracts of
P. angolensis
were prepared. The
phytochemical screening of the extracts was analyzed using standard method.
In vitro
cytotoxicity of the extract on HeLa cells
was evaluated using resazurin assay with the reference drug Emetine. Our result of the phytochemical screening revealed the
presence of glycosides, alkaloids, saponin, steroids, tannins, flavonoids and terpenoids. A value of p<0.05, <0.01 and <0.0001
were considered to be significant, very significant and highly significant, respectively. The extracts decreased the viability of
the cells in a concentration-dependent manner. The Ethyl Acetate extract of
P. angolensis
showed a significant cytotoxicity with
CC
50
90.27 ug mL
-1
. The Dichloromethane extract demonstrated a higher cytotoxic activity with CC
50
26.66 ug mL
-1
<30 ug mL
-1
a limit recommended for cytotoxicity for extract. The result of cytotoxicity study showed that
P. angolensis
extracts as having
potential inhibitory effect on HeLa cells. In conclusion, the dichloromethane and ethyl acetate extracts of
P. angolensis
are
promising cancer drug and their significance may increase in future in view of the lack of unwanted side effects characteristic
for Emetine compound currently in clinical use for treatment of cancer.
ijeomasolomonokoro@gmail.com