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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Use of gas chromatography to determine the cholesterol level in samples of meat and meat products
Ropota Mariana, Olteanu Margareta and Criste Rodica Diana
IBNA-Balotesti, Romania
Presently, consumers are increasingly attentive to the cholesterol concentration in animal foods. Hence, we used gas
chromatography to determine the cholesterol level in samples of broiler meat (breast and leg) and in various chicken
products (chicken frankfurters and chicken roll) and in pig meat (muscle, leg) and pig meat products (fillet). The method
we used is in agreement with AOAC International 2002-AOAC 994.10 standard. The samples were processed chemically by
saponification with 5% KOH in methanol, pH=2, extraction in petrol ether, concentration and dissolution in chloroform,
and were thereafter analysed by gas chromatography. We used a Perkin Elmer-Clarus 500 with flame ionization detector and
separation capillary HP-5, with hydrogen as carrier gas and air as burning gas. The method was validated “in house”, and used
as certified reference a standard chloroform solution, 10 mg/mL, SUPELCO, NIST traceable. We determined the following
parameters: accuracy, fidelity, repeatability, reproducibility, sensitivity, detection limit, quantification limit and recovery,
according to SR EN ISO/CEI 17025:2005, all values being within the admitted range. The following cholesterol concentrations
were thus determined in chicken meat and meat products: 150.96±1.45 g/100g breast meat; 164.63±0.72 g/100g leg meat;
71.77±1.35 g/100g frankfurters and 185.44±0.34 g/100g chicken roll; and in pig meat and meat products: 151.31±4.72 g/100g
muscle; 152.65±2.59 g/100g leg; 87.04±3.74 g/100g fillet.
Biography
Ropota Mariana finished her PhD in 2000 within the Faculty of Analytical Chemistry of the Bucharest University. She is coordinating the compartment of gas
chromatographic analyses within the Laboratory of Chemistry and Nutrition Physiology of the National research-Development Institute for Animal Biology and
Nutrition-IBNA-Balotesti. She published more than 20 papers in national and international scientific journals, rated by ISI or by other databases.
Ropota Mariana et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)