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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
A validated LC-MS/MS assay for the quantitative determination of hypophyllanthin and silibinin in
human plasma: Application to a pharmacokinetic study in healthy volunteers
Said A Hassan1 , Mohsen M Fathallah2, Medhat A Al-Ghobashy1, Eman G Nouman 2, Amira S Gouda3, Yasmine A Seif4, Mourad Aly4, Shaymaa M El-
Awady5 and Amgad I Alkorashy3
1Cairo University, Egypt
2Genuine Research Center, Egypt
3Al-Azhar University, Egypt
4Helwan University, Egypt
5Ain Shams University Hospitals, Egypt
Aselective and sensitive liquid chromatography tandem mass spectrometry method (LC-MS/MS) has been developed for
determination of Hypophyllanthin (HPT) and Silibinin (SBN) in human plasma. Sertraline hydrochloride was used as
the internal standard (IS). Sample preparation involved liquid–liquid extraction by tert butyl metyl ether. Chromatographic
separationwas carried out on a C8 column (3 μm, 3.0 x 50.0mm) with isocratic elution using amobile phase of water:acetonitrile
(10:90 v/v) at a flow rate of 0.6 mL/min. The detection was performed by tandem mass spectrometry with multiple reactions
monitoring mode via electrospray ionization source in positive ionization mode. Analysis was carried out within 2.0 min over
a linear concentration range of 1.00 -1000 and 1.00 – 500 ng/mL for HPT and SBN, respectively, and the LLOQ was 1 ng/ml for
both compounds. The method was validated according to FDA guidelines for bioanalytical method validation and satisfactory
results were obtained. This validated method was successfully applied to a pharmacokinetic study enrolling 20 male volunteers
administered a single oral dose of Heptex vegetable capsules.
Biography
Said A Hassan is a Lecturer of Analytical Chemistry and Instrumental Analysis at Faculty of Pharmacy, Cairo University. In 2015, he was awarded the PhD degree
in Analytical Chemistry. He finished the MSc in Analytical Chemistry in 2012 and was awarded the best master’s thesis in Faculty of Pharmacy, Cairo University. He
got the BSc in Pharmaceutical Science in Faculty of Pharmacy, Cairo University in 2007 graded Excellent with honor and ranked 8th in his class. He has experience
in topics such as UV-VIS spectrophotometry, chromatographic techniques, capillary electrophoresis, chemometrics & electrochemical methods of analysis.
Said A Hassan et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)